5S RNA Regulation and Ribosome Biogenesis

该研究旨在研究5S RNA的调控， 它是核糖体生物合成的一个组成部分。 在高等真核生物中，5S RNA是一种120 nt的小RNA，虽然它是细胞的重要组成部分， 核糖体，仍然只是部分了解其核糖体的位置 和功能 在体细胞中，5S RNA在细胞核中合成， 并靶向核仁，在那里它被并入新生的 更大的核糖体亚基。在卵母细胞中， 5S RNA的激活提前了几周，rRNA和RNA的激活也提前了几周。 蛋白质合成和核糖体生物合成的开始。因此，我们认为， 这种自然发生的同步在一组合成 核糖体成分为我们提供了一个独特的机会来研究各种 步骤和调节参与靶向的5S RNA到 核仁和5S RNA掺入的要求5S RNA是如何 在非洲爪蟾卵子发生过程中添加到新生的核糖体大亚基中 光滑。 另一个目的是建立一个或多个机制， 5S RNA进入细胞质，使其储存在那里， 将其运送回细胞核，特别是核仁。 在更大的背景下，卵母细胞系统将有助于我们理解 核蛋白如何与特定的RNA分子相互作用， 是RNP进出细胞核所必需的。 卵母细胞 研究可能会扩大我们对体细胞5S RNA靶向并整合到新生的 核糖体在核仁中形成，这一过程可能 完全发生在体细胞核中。

The research is directed toward studying the regulation of 5S RNA, which is one component of ribosome biogenesis. In higher eukaryotes, 5S RNA is small 120 nts RNA which, although an essential component of ribosomes, is still only partially understood in its ribosome location and function. In somatic cells 5S RNA is synthesized in the nucleus, and targeted to the nucleolus where it is incorporated into nascent larger ribosomal subunits. In oocytes, the synthesis of a large amount of 5S RNA precedes by several weeks, the activation of rRNA and r protein synthesis and the beginning of ribosome biogenesis. Therefore, this naturally occurring a synchrony in the on set of synthesis of ribosome components affords us a unique opportunity to study the various step sand the regulation involved in the targeting of 5S RNA to the nucleolus and the requirements for 5S RNA incorporation how 5S RNA is added to the nascent large ribosomal subunit during oogenesis in Xenopus laevis. A further aim is to establish the mechanism(s) for ferrying the 5S RNA out to the cytoplasm, keeping it stored there, and then transporting it back into the nucleus, specifically into the nucleolus. In a larger context, the oocyte system will facilitate our understanding of how nuclear proteins interact with specific RNA molecules, and what is required for transport of RNPs in and out of the nucleus. The oocyte studies will likely amplify our understanding of how somatic cell 5S RNAis targeted to and incorporated into nascent ribosomes forming with in the nucleolus, a process which probably occurs entirely with in the somatic cell nucleus.