Characterization of Metabolism and Excretion Patterns of Pulpmill Effluent Components by Fish

长期和中期研究：鱼类对纸浆厂废水成分代谢和排泄模式的表征。该奖项是在外国卓越中心长期和中期研究计划下推荐的。该项目旨在使年轻的美国科学家和工程师能够在国外卓越的研究机构进行长期研究。该奖项为开展联合研究以及利用国外独特或互补的设施、专业知识和实验条件提供了机会。该奖项将支持田纳西大学的Marsha Black博士和芬兰约恩苏大学的Aimo Oikari博士进行为期12个月的研究访问。研究人员将采用分层方法，利用实验室和现场暴露来研究两种纸浆厂废水成分（脱氢枞酸和3,4,5-三氯甲酚）和苯并a！芘经鱼鳃、皮肤、肾脏和肝胆系统排出。鳟鱼的复合排泄模式和代谢物形成率将使用实验箱进行量化。模型化合物的排泄将在预先暴露于稀释的纸浆厂废水或>-napthoflavone（一种单加氧酶系统的有效诱导剂）的情况下和不事先暴露于稀释纸浆厂废水的情况下进行表征。在受控条件下进行这些实验室实验后，白鱼将被关在离污水排放区3-15公里的一个接收纸浆厂污水的湖中。在6个月的暴露期内，每隔一段时间取出的鱼将被分析其代谢酶活性和脱氢枞酸和3,4,5-三氯guiacol的代谢物。实验室和实地研究的结果将用于评价外来生物排泄在鱼类适应慢性低水平污染方面的作用。该奖项建议提供资金，酌情支付国际旅行，国外本地旅行，津贴，家属津贴（如果适用），以及美国本土机构的固定行政津贴250美元。

Long and Medium Term Research: Characterization of Metabolism and Excretion Patterns of Pulpmill Effluent Components by Fish. This award recommendation is made under the Program for Long and Medium-Term Research at Foreign Centers of Excellence. The program is designed to enable young U.S. scientists and engineers to conduct long-term research abroad at research institutions of proven excellence. Awards provide opportunities for the conduct of joint research and the utilization of unique or complementary facilities, expertise and experimental conditions in foreign countries. The award will support a 12 month research visit by Dr. Marsha Black, University of Tennessee, with Dr. Aimo Oikari of the University of Joensuu, Finland. The researchers will use a tiered approach using both laboratory and field exposures to study the elimination of two pulpmill effluent components (dehydroabietic acid and 3,4,5- trichloroguiacol) and benzo?a!pyrene by fish via the gills, skin, kidney, and hepatobiliary system. Compound excretion patterns and rates of metabolite formation by trout will be quantified using an experimental chamber. Excretion of the model compounds will be characterized with and without preexposure to dilute pulpmill effluent or >-napthoflavone, a potent inducer of the monooxygenase enzyme system. Following these laboratory experiments under controlled conditions, whitefish will be caged in a lake receiving pulpmill effluents at locations 3-15 km from the effluent discharge area. Fish removed at specified intervals over the 6 month exposure period will be analyzed for metabolizing enzyme activities and metabolites of dehydroabietic acid and 3,4,5-trichloroguiacol. Results from laboratory and field studies will be used to evaluate the role of xenobiotic excretion in the adaptation of fish to chronic low-level pollution. The award recommendation provides funds to cover, as appropriate, international travel, local travel abroad, stipend, dependents' allowance, if applicable, and a flat administrative allowance of $250.00 for the U.S. home institution.