Metabolic Pathway Engineering of the 1,3 - Propanediol System

代谢途径工程(MPE)是指设计、分析和构建生化途径，是一项对化学品生产和有毒化合物降解具有广泛影响的新兴技术。MPE包括将遗传物质添加到宿主有机体中，以修改现有的途径或创建新的途径。该项目的目标是通过在大肠杆菌中构建一种新的途径来直接解决与MPE相关的关键问题。本研究选择的模型途径是肺炎克雷伯氏菌进行的甘油转化为1，3-丙二醇。第一步是表征肺炎克雷伯菌的碳通量和胞内中间浓度，作为与工程菌比较的基础。最后一步是研究新途径在大肠杆菌中的功能，并研究其与宿主代谢的相互作用。这项工作有望为MPE的研究提供新的视角，并为进一步的研究提供一个具有良好特色的模型体系。此外，1，3-丙二醇是一种有用的化学中间体，这项工作可能会导致其生产的改进。

Metabolic Pathway Engineering (MPE), defined as the design, analysis, and construction of biochemical pathways, is an emerging technology with broad implications for the production of chemicals and the degradation of toxic compounds. MPE involves adding genetic material to a host organism to modify an existing pathway or to create a new one. The objective of this project is to directly address key problems associated with MPE by constructing a new pathway in E. coli. The model pathway selected for this research is the conversion of glycerol to 1,3 - propanediol as carried out by Klebsiella pneumoniae. The first step is to characterize the carbon flux and intracellular intermediate concentrations of K. pneumoniae, as a basis of comparison with the engineered E. coli. The final step is to then study the function of the new pathway in E. coli and investigate its interactions with the host metabolism. This work is expected to provide new insight into MPE and to provide a well characterized model system for further research. In addition, 1,3 - propanediol is a useful chemical intermediate, and it is possible that this work will lead to improvements in its production.