Development of Electrical Properties in Neural-Lineage Cellsof Early Xenopus Embryos

该项目将研究电生理学的发展 早期非洲爪蟾胚胎神经细胞的特性。 虽然很多注意力都集中在调制 终末分化过程中的离子通道群 有丝分裂后神经元的特定集合， 是关于离子通道人口的发展， 早期阶段的神经系细胞， 神经表型的变化 穆迪博士将使用膜片钳技术来研究这个问题 在非洲爪蟾胚胎中，在未受精卵母细胞 和神经胚 他将研究细胞在哪些阶段 参与胚胎神经系统的形成 表达功能性离子通道群体，其不同于 其他的命运，然后研究发展过程， 卵母细胞的电特性被改变 转化为神经元表型的细胞。 特别 将注意力放在离子通道合成的作用， 预先存在的信道的调制，以及空间分布 早期单细胞表面的离子通道 神经系统的电生理分化。

This project will examine the development of electrophysiological properties in neural-lineage cells of the early Xenopus embryo. Although much attention has focussed on the modulation of populations of ion channels during the terminal differentiation of specific sets of post-mitotic neurons, very little information is available about the development of ion channel populations in neural-lineage cells at earlier stages, when initial commitment to neural phenotypes occurs. Dr. Moody will use patch clamp techniques to study this problem in the Xenopus embryo, at stages between the unfertilized oocyte and the neurula. He will examine the stages at which cells involved in the formation of the embryonic nervous system first express functional ion channel populations distinct from cells of other fates, and then investigate the developmental processes by which the electrical properties of the oocyte are transformed into those of cells committed to neuronal phenotypes. Particular attention will be paid to the roles of ion channel synthesis, the modulation of pre-existing channels, and the spatial distribution of ion channels over the surface of single cells in the early electrophysiological differentiation of the nervous system.