SGER: Development of Ion Channels in Mouse Ventricular Zone Cells

SGER：小鼠心室区细胞离子通道的发育

• 批准号: 9708656
• 负责人: William Moody
• 金额: $ 4.22万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9708656&HistoricalAwards=false
• 关键词: SGER; Development; Ion; Channels; Mouse

IBN-9708656 PI: MOODY The neurons that populate the mammalian brain are generated during embryonic development from cells that line the ventricles. These cells divide repeatedly, and then migrate away from the ventricles to populate the brain itself. It is critically important that the processes of division and migration occur correctly in order for the brain to develop normally. Although it is well known that mature neurons in the brain use electrical signalling to process information, there is also some evidence that modified forms of electrical signalling are used during the processes of division and migration that create the brain during early embryogenesis. Dr. Moody will use quantitative techniques to record electrical signals from individual cells lining the ventricles of the embryonic mouse brain to determine how such signals are used in early brain development. By using living slices of embryonic brain tissue, it will be possible to identify individual cells according to their state of division and migration, and to then detemine the exact nature of the electrical signals they can generate. Once this is understood, specific drugs that block different aspects of those signals will be used to determine whether normal patterns of division and migration require this type of signalling. Results from this project will allow us to understand whether the normal adult forms of electrical communication between neurons in the brain are also used in modified form to mediate critical aspects of neural development.

IBN-9708656 PI：穆迪，哺乳动物大脑中的神经元是在胚胎发育过程中从排列脑室的细胞中产生的。这些细胞反复分裂，然后从脑室迁移到大脑本身。为了大脑的正常发育，正确地进行分裂和迁移过程是至关重要的。尽管众所周知，大脑中的成熟神经元使用电信号来处理信息，但也有一些证据表明，在早期胚胎发育期间创造大脑的分裂和迁移过程中使用了修改后的电信号形式。穆迪博士将使用定量技术记录小鼠胚胎脑室内单个细胞发出的电信号，以确定这些信号在早期大脑发育中的作用。通过使用活的胚胎脑组织切片，可以根据细胞的分裂和迁移状态来识别单个细胞，然后确定它们可以产生的电信号的确切性质。一旦了解了这一点，将使用阻断这些信号不同方面的特定药物来确定正常的分裂和迁移模式是否需要这种类型的信号。这个项目的结果将使我们了解大脑中神经元之间正常的成人形式的电通信是否也以修改的形式用于调节神经发育的关键方面。