US-Poland Research on Escherichia Coli Heat Shock Proteins

这项由犹他大学的Constantine Georgopoulos博士和格但斯克大学的Maciej Zylicz博士合作开展的美国-波兰研究项目的主要目标是研究三种大肠杆菌热休克蛋白的分子作用机制。这些蛋白质在噬菌体lambda复制中的作用将在胁迫和非胁迫条件下研究，方法包括蛋白质纯化和蛋白质相互作用的直接生化分析。结果应该有助于我们了解基本蛋白质的理化性质和作用方式。在这个过程中，研究人员希望测试有关蛋白质与其他蛋白质的疏水区域结合以及防止或破坏某些蛋白质之间相互作用的一般假设。该生物化学项目通过使美国和东欧的领先专家在共同感兴趣和能力强的领域结合互补的人才和集中研究资源，实现了推进科学知识的计划目标。

The primary objective of this U.S.-Poland research project between Dr. Constantine Georgopoulos of the University of Utah and Dr. Maciej Zylicz of the University of Gdansk is to examine the molecular mechanisms of action in three E. coli heat shock proteins. The role of these proteins in replication of the bacteriophage lambda will be studied under stree and non-stress conditions using approaches involving protein purification and direct biochemical analysis of protein interaction. Results should contribute to our understanding of basic protein physiochemical properties and modes of action. In the process, the researchers expect to test general hypotheses concerning protein involvement in binding to hydrophobic domains of other proteins and preveting or disrupting certain protein-protein interactions. This project in biochemistry fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents and pool research resources in areas of strong mutual interest and competence.