Structure and Function of Proline Endopeptidase

本研究旨在确定 脯氨酸内肽酶，一种普遍存在的细胞成分。 这种酶在蛋白质分解中起着独特的作用， 对于具有以下结构的丙基残基的特殊特征， 破坏阿尔法螺旋的特性。 这种酶的一种形式是 膜结合并在分离的突触体中积累， 脊椎动物的大脑 因此，这种酶有可能 在神经组织的蛋白质分解中起着特殊的作用。 本项目的目标如下：1）确定 通过分离和序列分析确定酶的结构; 2)以确定对以下物质至关重要的特定残留物： 酶活性和3）确定哪些因素参与 非活性形式的酶的健康介导的转化。

This research is designed to determine the structure of the enzyme proline endopeptidase, an ubiquitous cell constituent. The enzyme plays a unique role in the breakdown of proteins due to the special feature of the propyl residues which have the properties of breaking alpha helices. One form of this enzyme is membrane bound and accumulates in synaptosomes isolated from vertebrate brain. It is possible, therefore, that the enzyme has a particular role in protein breakdown in the nervous tissue. The goals of this project are as follows: 1) to determine the structure of the enzyme through isolation and sequence analysis; 2) to identify the specific residues which are critical for enzymatic activity and 3) determine which factors are involved in the heath-mediated conversion of the enzyme in an inactive form.