RUI: Analysis of Pronucleus Motility in Marine Eggs

在真核细胞内运动的基本事件中 是细胞核和细胞器的定向运动， 与微管或微丝结合。 而 微丝的单向运动是由 ATP酶肌球蛋白，微管上的双向运动， 由两个不同的马达调节。 所述蛋白质驱动蛋白可以 作为一个顺行性细胞器转运，而 类似于睫状动力蛋白的蛋白质可能具有逆行功能 易位蛋白 建议通过以下方式研究该机制： 海洋卵的原核沿着微管运动。 这些卵子很容易获得，可以很容易地受精， 开始分裂，非常适合逆行的研究 能动性 雌性原核完全以 沿着沿着微管的逆行方向， 精子星，而其他细胞器的运动占主导地位，但不是 在这个方向上。 体外易位系统 将被构建来观察原核的运动。 的 负责这些运动的逆行转位器将是 定位和表征，以及其他细胞质因子 将确定逆行运动的必要条件。 最后， 运动的调节和变化之前发生的， 受精后将进行调查。 这些研究的结果将增加我们对 细胞器移动的机制， 特别是，将表征原核的运动 受精卵

Among basic events of intracellular motility in eukaryotic cells are directed movements of nuclei and organelles occurring in association with microtubules or microfilaments. While unidirectional movements on microfilaments is mediated by the ATPase myosin, bidirectional movements on microtubules are mediated by two different motors. The protein kinesin may function as an anterograde organelle translocator, while a protein similar to ciliary dynein may function as a retrograde translocator. It is proposed to investigate the mechanism by which the pronuclei of marine eggs move along microtubules. These eggs are readily available, can be easily fertilized to initiate division, and are well suited for studies of retrograde motility. The female pronucleus moves exclusively in a retreograde direction along the microtubules assembled by the sperm aster, while other organelles move predominantly, but not exclusively, in this direction. An in vitro translocation system will be constructed to observe movements of pronuclei. The retrograde translocator responsible for these movements will be localized and characterized, and other cytoplasmic factors necessary for retrograde movements will be identified. Finally, the regulation of motility and changes that occur before and after fertilization will be investigated. The results of these studies will increase our understanding of the mechanisms by which cellular organelles move, and, in particular, will characterize the the movement of the pronuclei of fertilized eggs.