Enzymes in the D-Alanine Branch of Bacterial Cell Wall Peptidoglycan Assembly

这项更新建议涉及的酶， 提供并掺入D-丙氨酸（D-ala）和二肽， D-Ala-D-Ala进入细胞壁的肽聚糖层， 细菌 三种酶组成D-丙氨酸分支，丙氨酸 消旋酶、D-Ala-D-Ala连接酶和D-Ala-D-Ala加成酶。 在过去的资助期内，来自大肠杆菌和 将鼠伤寒沙门氏菌纯化至同质， 首次 在新的资助期内将致力于研究 每个基因的机制、结构/功能以及 冷酶 此外，第一种酶在 肽聚糖途径，UDP-N乙酰葡糖胺 将烯醇化酰基转移酶纯化并表征。 这一结果将大大加深我们对hw的理解 细菌生长并建造细胞壁。 它们也可能导致 设计有效化疗药物的新策略 防止细菌感染。

This renewal proposal deals with the enzymes involved in providing and incorporating D-alanine (D-ala) and the dipeptide D-Ala-D-Ala into the peptoglycan layer of cell walls in bacteria. Three enzymes comprise the D-Alanine branch, alanine racemase, D-Ala-D-Ala ligase and the D-Ala-D-Ala adding enzyme. In the past grant period (s) all three enzymes from E.coli and Salmonella typhimurium were purified to homogeneity for the first time. In the new grant period will be devoted to the study of the mechanism, structure/function of each of the genes and the encolded enzymes. In addition, the first enzyme in the peptoglycan pathway, the UDP-Nacetylglucosamine enolpyruvyltransferase will be purified and characterized. The results will greatly enchance our understanding of hw bacteria grow and build their cell walls. They may also lead to new strategies for designing effective chemotherapeutic agents against bacterial infections.