Molecular and Genetic Characterization of the Drosophila Trans-acting Factor Elf-1

这项研究的最终目的是阐明 在DNA结合因子Elf-1发育过程中的作用。 这 因子与序列元件相互作用，该序列元件特异性地 多巴脱羧酶基因在细胞中表达所需的 果蝇的中枢神经系统 的首要目标 建议是没有使用两种不同的方法来操纵 在体内表达Elf-1，并分析这些因素的影响 对多巴脱羧酶表达的操作，以及对 表达的基因Ultrabithorax和enrailed其中Elf- 1、绑定。 一种方法是将突变分离出来， Elf-1基因和另一个基因在大肠杆菌中异位表达蛋白。 紧张。 初步实验已经证实 三种不同的Elf-1亚型，因此研究的第二个目的是 是研究变异体的总数和结构 Elf-1蛋白，并确定其意义 变化量 这将涉及比较活动和 不同形式的分布。 本提案中的工作应增进我们对以下问题的理解： 不同基因选择性转化的机制 在不同类型的组织中时断时续

The ultimate aim of the proposed research is to elucidate the role during development of the DNA-bind factor Elf-1. This factor interacts with a sequence element which is specifically required for expression of the dopa decarboxylase gene in the central nervous system of Drosophila. The first aim of proposal is no use two different approaches to manipulate the expression of Elf-1 in vivo and analyse the affect of these manipulations on dopa decarboxylase expression, and on the expression of the genes Ultrabithorax and engrailed which Elf- 1 also binds. One approach is to isolate mutations in the Elf-1 gene and the other to express the protein ectopically in transformant strains. Preliminary experiments have identified three different Elf-1 isoforms so a second aim of the research is to investigate the total number and structure of the variant Elf-1 proteins, and to determine the significance of this variation. This will involve comparing the activities and distribution of the different forms. The work in this proposal should increase our understanding of the mechanisms by which different genes are selectively turned on and off in different types of tissues.