Enzymes Involved in Plant Cell Wall Synthesis

该项目采用了分馏，蛋白质 标记和抗体技术来鉴定和纯化 豌豆高尔基体多肽组分和质膜葡聚糖 合成酶系统 这项工作使鉴定成为可能 并克隆相关基因序列， 植物细胞壁合成和高尔基体的调节方面 功能可以进一步研究。 细胞壁多糖通常是最丰富的 植物新陈代谢的产物。 它们的生物合成是 作为结构蛋白，是植物生长的重要特征 合成对动物的生长是有好处的。 墙壁合成必须是 与细胞增大相关;在生长的组织中， 受生长刺激激素的调节， 环境信号。 墙体合成也是一个突出的方面 植物细胞的分化。 综合指数显著上升- 在厚壁专业化的发展过程中， 细胞如表皮细胞、维管元件、纤维和 石细胞 因此，了解墙的控制 合成多糖的酶和编码 它们将使我们更接近植物细胞生物学的核心， 发展

This project employs a combination of fractionation, protein labeling, and antibody techniques to identify and purify polypeptide components of pea Golgi and plasma membrane glucan synthase systems. This work makes possible the identification and cloning of the responsible gene sequences so that molecular aspects of the regulation of plant cell wall synthesis and Golgi function can be further investigated. Cell wall polysaccharides are generally the most abundant products of plant metabolism. Their biosynthesis is as characteristic and central to plant growth as structural protein synthesis is to the growth of animals. Wall synthesis must be correlated with cell enlargement; in growing tissues it is up- regulated in response to growth-stimulating hormones and environmental signals. Wall synthesis is also a prominent aspect of plant cell differentiation. Synthesis is conspicuously up- regulated during the development of thick-walled specialized cells such as epidermal cells, vascular elements, fibers and sclereids. Thus, understanding the control of wall polysaccharide-synthesizing enzymes and the genes that encode them would bring us close to the heart of plant cell biology and development.