Structure-function relationship of endo-type enzymes involved in plant cell surface proteoglycans

植物细胞表面蛋白多糖内切酶的结构与功能关系

• 批准号: 22580110
• 负责人: KANEKO Satoshi
• 金额: $ 3万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580110/
• 关键词: 酵素利用学; 糖質関連酵素; アラビノガラクタン-プロテイン; アラビノガラクタン; 糖加水分解酵素; 結晶構造解析; 構造機能相関; エンド-1,6-ガラクタナーゼ; Streptomyces avermitilis; エンド-β-1,6-ガラクタナーゼ; Streptomyces avemitilis

An endo-.-1,6-galactanase which act on thickening agent arabinogalactan was obtained from Streptomyces avermitilis. The substrate specificity and the crystal structure of the recombinant enzyme were elucidated. To understand the detail mechanism of substrate specificity of the endo-.-1,6-galactanase, the other endo-.-1,6-galactanase which consist from distinctive amino acid sequence was cloned and crystal of the recombinant enzyme was obtained. However, quality of the crystal was not enough to solve structure so that the mechanism of substrate specificity of the endo-.-1,6-galactanase could not be elucidated.

一个endo-.-从阿维链霉菌（Streptomycesavermiatum）中分离得到1，6-半乳聚糖酶，该酶作用于增稠剂阿拉伯半乳聚糖。对重组酶的底物特异性和晶体结构进行了鉴定。为了了解内切酶底物特异性的详细机制，1，6-半乳聚糖酶，另一种内切酶。克隆了由不同氨基酸序列组成的1，6-半乳聚糖酶，并获得了重组酶的晶体。然而，晶体的质量不足以解决结构问题，因此内切酶的底物特异性机制尚不清楚。1，6-半乳聚糖酶不能被解析。

项目成果

Characterization of a α-L-rhamnosidase from Streptomyces avermitilis

阿维链霉菌 α-L-鼠李糖苷酶的表征

• 发表时间: 2013
• 期刊: Biosci. Biotechnol. Biochem
• 作者: Hitomi Ichinose;Zui Fujimoto;and Satoshi Kaneko
• 通讯作者: and Satoshi Kaneko

The Structure of a Streptomyces avermitilis α-L-Rhamnosidase Reveals a Novel Carbohydrate-binding Module CBM67 within the Six-domain Arrangement

• DOI: 10.1074/jbc.m113.460097
• 发表时间: 2013-04-26
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Fujimoto, Zui;Jackson, Adam;Kaneko, Satoshi
• 通讯作者: Kaneko, Satoshi