Presidential Young Investigator Award: Role of Nucleic Acid Conformation in Genetic Regulation

为了了解核酸构象在 基因调控，Feigon博士计划：1）继续研究 含有潜在B-Z连接和三联体的DNA寡聚体 链DNA; 2）研究棘霉素-DNA复合物; 3） 研究ECORI限制酶和甲基化酶如何结合 特异性地针对DNA序列GAATTC;以及4）该研究 色氨酸阻遏物与操纵基因DNA的相互作用。 该项目关注的是构象的范围， 核酸采用，无论是在溶液中游离，并在复合物与 蛋白质和药物，以及决定 蛋白质和其它配体的序列特异性结合 核酸 该项目的主要研究工具是 高场核磁共振光谱学，特别是 二维的，来阐明结构和动力学。

In order to understand the role of nucleic acid conformation in genetic regulation, Dr. Feigon plans to: 1) continue studies of DNA oligomers containing potential B-Z junctions and triple stranded DNAs; 2) investigate echinomycin-DNA complexes; 3) examine how ECORI restriction enzyme and methylase bind specifically to the DNA sequence GAATTC; and 4) the study the interaction of the typtophan repressor with operator DNA. This project is concerned with the range of conformations that nucleic acids adopt, both free in solution and in complex with proteins and drugs, and in the specific factors that determine sequence specific binding of proteins and other ligands to nucleic acids. The primary research tool for this project is high field nuclear magnetic resonance spectroscopy, particulary two-dimensional, to elucidate structure and dynamics.