High-Resolution Preparative Liquid Chromatography

这个小型企业创新研究(SBIR)第一阶段项目是在分离科学的一般领域和子领域的液相色谱学。这项研究的重点是设计、建造和评价一种新型的能够高分辨率分离克量的多肽激素和其他物质的色谱系统。该系统的核心是一种商用的挤压多通道陶瓷块，具有数千个相同尺寸的平行通道。这相当于并行使用数千个相同的色谱柱。因此，毛细管柱的拆分能力可以与制备规模柱的较高样品容量相结合。第一阶段的具体任务包括开发一种干式包装陶瓷块的方法，构建包含这些块的整个色谱系统，以及使用荧光碳氢化合物测试化合物对系统进行评估。该项目的成功将是分离技术向前迈出的重要一步。预计投影制备毛细管柱技术的发展将对生物技术分离产生重大影响。

This Small Business Innovation Research (SBIR) Phase I project is in the general area of separations science and in the subfield of liquid chromatography. The thrust of this research is the design, construction, and evaluation of a new type of chromatographic system capable of separating gram quantities of peptide hormones and other substances with high resolution. The heart of this system is a commercially available, extruded multichannel ceramic block having several thousand parallel channels of identical dimensions. This is equivalent to using thousands of identical chromatographic columns in parallel. Thus, the resolving power of a capillary column can be combined with the higher sample capacity of a preparative scale column. Specific Phase I tasks include development of a method for dry-packing the ceramic blocks, construction of an entire chromatographic system incorporating these blocks, and evaluation of the system using fluorescent hydrocarbon test compounds. Success of this project will be a major step forward in separations technology. It is expected that the development of the projected preparative capillary column technique could have major impact of biotechnological separations.