Preparative Liquid Chromatography Equipment for Fundamental Studies on Protein Purifications

用于蛋白质纯化基础研究的制备型液相色谱设备

• 批准号: 9007785
• 负责人: Nien-Hwa Wang
• 金额: $ 2万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9007785&HistoricalAwards=false
• 关键词: Preparative; Liquid; Chromatography; Equipment; Fundamental

The Principal Investigator (PI) proposes to carry out fundamental studies to characterize intrinsic kinetics and adsorption parameters for proteins in ion exchange and affinity chromatography systems. The PI also proposes to establish the methodology for designing large-scale chromatography for proteins. Several model proteins which have well characterized molecular weights, isoelectric points, amino acid sequences, and three dimensional structures will be studied. Computer simulations which have been previously developed for isocratic operations will be extended to gradient elution systems. Nonlinear isotherms, size exclusion, mass transfer resistances, slow adsorption-desorption kinetics, and other time-dependent adsorption phenomena such as protein aggregation and conformational changes will be considered in the model. The model and simulations will be validated with column experiments on ion exchange and affinity chromatography. After validation, the simulations can be used for designing more efficient and cost-effective processes by choosing appropriate design and operating parameters, such as buffer pH, types of buffer, salt concentration, feed size, particle size, and flow rate.

主要研究者（PI）建议开展 表征本征动力学的基础研究， 离子交换中蛋白质的吸附参数， 亲和层析系统。 PI还建议 建立设计大型 蛋白质的层析。 几种模型蛋白， 具有良好表征的分子量、等电性 点、氨基酸序列和三维 将研究结构。 计算机模拟， 先前已开发用于等度操作 将扩展到梯度洗脱系统。 非线性 等温线，尺寸排阻，传质阻力，慢 吸附-脱附动力学和其他时间依赖性 吸附现象，如蛋白质聚集和 在模型中将考虑构象变化。 模型和模拟将通过列验证 离子交换和亲和层析实验。 经过验证，仿真结果可用于设计 更高效、更具成本效益的流程， 适当的设计和操作参数，如缓冲液 pH、缓冲液类型、盐浓度、进料量， 粒度和流速。