PRF/J: DNA Sequence Analysis and the Phylogeny of the Liliopsida (Monocotyledoneae)

叶绿体基因的DNA核苷酸碱基序列将用于解释百合亚类（即单子科）之间的系统发育关系。这个基因编码核酮糖1,5-二磷酸羧化酶（rbcL）的大亚基，这是地球上最丰富的，也可以说是最重要的蛋白质的一部分。加州大学河滨分校（University of California, Riverside）遗传学教授迈克尔·克莱格（Michael Clegg）博士已同意赞助这项奖学金提案。rbcL基因是一个特别合适的选择，因为它在光自养生物中无处不在，因为它表现出足够的分化，可以用于广泛的系统发育研究，因为rbcL核苷酸序列数据库已经存在，将与序列数据一起使用。现代测序技术允许一个研究者在短时间内对许多物种进行分析，特别是在常规进行序列分析的实验室里，比如克莱格博士。总细胞DNA将被提取，rbcL基因将在聚合酶链反应中扩增，核苷酸序列将通过标准技术确定。预计本研究将需要两年的工作和支持，其中14-16个月用于植物材料的采购和rbcL基因序列的确定，其余时间将用于数据分析和系统发育解释。

The DNA nucleotide base sequence of a chloroplast gene will be used to interpret phylogenetic relationships among the subclasses of the Liliopsida (i.e., the monocots). This gene encodes the large subunit of ribulose 1,5-bisphosphate carboxylase (rbcL), part of the most abundant, and arguably, the most important protein on earth. Dr. Michael Clegg, Professor of Genetics at the University of California, Riverside, has agreed to sponsor this fellowship proposal. The rbcL gene is a particularly appropriate choice because it is ubiquitous among photoautotrophs, because it exhibits sufficient divergence for use in broad phylogenetic investigations, and because a data base of rbcL nucleotide sequences already exists which will be used in conjunction with sequence data. Contemporary sequencing technology permits the analysis of many species in a short period of time by one investigator, especially in a laboratory in which sequence analysis is conducted routinely, such as that of Dr. Clegg. Total cellular DNA will be extracted, the rbcL gene will be amplified in the polymerase chain reaction, and the nucleotide sequence will be determined by standard techniques. Sequence data will be analyzed to construct statistically supported dendrograms, It is anticipated that this research will require two years of work and support: 14-16 months will be spent procuring plant materials and determining rbcL gene sequences, and the remaining time will be devoted to analysis of the data and phylogenetic interpretation.