Protein-Membrane Organization and Function: Gramicidin Channel

本研究项目的长期目标有两个方面：（1） 为了获得离子通道系统如何 在离子选择模式下有效地起作用，以及（2） 发展实验技术和理论解释 可以应用于更复杂的生物学研究， 未来的交通系统。 多核-多维NMR光谱，连同 肽合成将用于研究单一的 短杆菌肽转运系统中的氨基酸取代。 这种替代对通道结构的影响， 将短杆菌肽整合到膜中，在通道上 形成，并对阳离子结合和运输将观察 和表征了

The long term goals of this research project are two fold: (1) to obtain a concise description of how an ion channel system functions efficiently in an ion selective mode and (2) to develop experimental techniques and theoretical interpretations that can be applied to the study of more complex biological transport systems in the future. Multinuclei-multidimensional NMR spectroscopy, together with peptide synthesis will be used to study the effect of single amino acid substitution in the gramicidin transport system. The effect of this substitution on channel structure, on the integration of the gramicidin into a membrane, on channel formation, and on cation binding and transport will be observed and characterized.