Genetic and Molecular Analysis of Development Caenorhabditis Elegans

调控信息从一种细胞或组织类型传递到 另一个是在多细胞生物的发育过程中。 线虫的发展，秀丽隐杆线虫， 在细胞水平上详细描述，并包括几个 调节性细胞间相互作用决定细胞 命运本建议调查的机制，两个 这种调节相互作用：诱导有丝分裂的胚芽， 早期咽肌细胞的分化和诱导 胚胎这两个事件发生在不同的时间， 不同的组织，但有一个共同的组成部分：GLP-1基因。 广泛的遗传和分子研究表明，GLP-1的行为， 作为每个诱导组织中的信号受体。缅因州医生 已经产生了GLP-1突变的基因外抑制因子， 识别负责信号产生的基因， 诱导组织和诱导组织中的信号转导。 该提案概述了遗传和分子研究以及细胞 烧蚀实验，旨在分析的作用（S） 这些在生殖细胞系和胚胎细胞中新发现的基因， 细胞相互作用特别是，该提案侧重于一个 一个被鉴定为隐性抑制基因sog-1， 与GLP-1相关的显性抑制因子。 %%% 这项研究旨在研究细胞命运如何 在多细胞生物的发育过程中被指定。 特别是，实验将集中在细胞-细胞 相互作用，需要指定的命运 当单个细胞分化成一个或另一个成体时 细胞类型。

Regulatory information is passed from one cell or tissue type to another during the development of multicellular organisms. Development of the nematode, Caenorhabditis elegans, has been described in detail at the cellular level and includes several instances where regulatory cell-cell interactions decide cell fate. This proposal investigates the mechanisms underlying two such regulatory interactions: induction of mitosis in the germ line and induction of pharyngeal muscle cells in the early embryo. These two events occur at different times and in different tissues but share a common component: the glp-1 gene. Extensive genetic and molecular studies suggest that glp-1 acts as a signal receptor in each of the induced tissues. Dr. Maine has generated extragenic suppressors of glp-1 mutations as a way of identifying genes responsible for signal production in the inducing tissue and signal transduction in the induced tissue. This proposal outlines genetic and molecular studies and cell ablation experiments that are designed to analyze the role(s) of these newly identified genes in the germ line and embryonic cell- cell interactions. In particular, the proposal focuses on one gene identified as a recessive suppressors, sog-1, and on a set of dominant suppressors that are linked to glp-1. %%% The proposed studies are designed to investigate how cell fate is specified during the development of multicellular organisms. In particular, the experiments will focus on the cell-cell interactions that are reguired to specify the fates of individual cells as they differentiate into one or another adult cell type.