Molecular Structure and Function Relations of Transients. Photoreceptors: Time-Resolved Infrared Studies

该建议旨在提供基本的见解， 光敏色素受体（植物生长和光响应 控制剂）和细菌视紫红质（光驱动质子 泵），蛋白质结构与蛋白质功能相关， 反应性 本研究的总体目标是研究，使用 皮秒至千秒时间分辨红外光谱 时间制度，光诱导的构象变化和动力学 通过遵循蛋白质酰胺I的动力学和 II区、羧基、特定氨基酸（即 色氨酸和酪氨酸）和蛋白质光中间体。 在 除了动态地理解 光和光敏的胆三烯光敏色素， 调查者将确定a）方向和强度 亚胺氢和附近的 抗衡离子;和B）附近的正电荷或负电荷的效应 双三烯质子化后的C=N频移 发色团和视网膜席夫碱发色团。 这 建议还旨在了解如何可逆光控 光敏色素和细菌视紫红质的性质可用于 生产光生物传感器或光电器件。

This proposal seeks to provide fundamental insight into how, in the phytochrome photoreceptor (the plant growth and photoresponse control agent) and bacteriorhodopsin ( a light driven proton pump), protein structure relates to protein function and reactivity. The overall goal of this research is to study, using time-resolved infrared spectroscopy from picosecond to kilosecond time regimes, light-induced conformational changes and kinetic intermediates by following the dynamics of the protein amide I and II regions, carboxylic groups, specific amino acid (i.e. tryptophan and tyrosine) and protein photointermediates. In addition to the dynamic understanding of the interaction between light and the photosensitive bilitriene phytochrome, the investigatior will determine a) orientation and strenght of possible hydrogen bonds between the imine hydrogen and a nearby counter ion; and b) effects of a positive or negative charge near the C=N frequency shift upon protonation of both the bilitriene chromophore and the retinal Schiff's base chromophore. This proposal is also aimed at learning how the reversible photocontrol properties of phytochrome and bacteriorhodopsin can be used to produce light biosensors or photodevices.