Microelectrode and Tracer Studies of the Chambered Nautilus Siphuncle

鹦鹉螺（Nautilus spp.）是唯一幸存的 曾经占优势的外腔头足类动物的属。 Nautilus在海水中实现了接近中性的水溶性， 它沉重的保护壳里充满气体的舱室。 的 虹吸管，通过动物的器官 腔室，去除最初填充的照相机液体 腔室，并允许气体（主要是N2）进入。 因此 虹吸管是鹦鹉螺浮力的器官。 为了了解虹吸管是如何排空和部分填充的 室，研究这种运输和电生理学， 必需的. 在撰写正式提案之前， 微电极和助焊剂实验，初步研究是 需要表明，适当的测量可以 在体外的管形细胞上制造的。 该SGER提案将 我们试图证明这些方法的可行性。 这项SGER提案是探索性的，风险很高，因为在 从未对管蚜进行过体外研究， 并不意味着虹吸管是一个有用的系统， 微电极和示踪剂实验。

The chambered nautilus (Nautilus spp.) is the sole surviving genus of the once dominant externally-chambered cephalopods. Nautilus achieves its near-neutral buoyance in seawater via the gas-filled chambers of its heavy protective shell. The siphuncle, the organ that passes through the animal's chambers, removes the cameral liquid which initially fills the chambers, and allows gas (mostly N2) to enter. Thus, the siphuncle is the organ responsible for nautilus' buoyancy. To understand how the siphuncle empties and partially fills chambers, studies of this transport and electrophysiology and required. Prior to writing a formal proposal involving microelectrode and flux experiments, preliminary studies are required to show that the appropriate measurements can be made on siphuncular cells in vitro. This SGER proposal will attempt to demonstrate the feasibility of those methods. This SGER proposal is exploratory and high risk because in vitro studies of the siphuncle have never been done and there is no quarantee that the siphuncle is a useful system for microelectrode and tracer experiments.***