RUI: Fate of Xenografts in Biomphalaria glabrata (Mollusca: Pulmonata)

当从一种软体动物身上移植组织时， 另一种（异种移植物），据报道移植物失败，即，死的 的 这项研究的目的是了解 软体动物异种移植失败。 具体来说，该研究将评估 （1）生理不相容性之间的相对作用 供体和受体;（2）免疫排斥反应， 收件人。 方法将包括植入心脏， 近缘种和远缘种 一个单一的受体蜗牛物种 植入物的命运 随后进行组织学和组织化学，并将比较 体外心脏的命运，以及植入物的命运 来自同一物种的捐赠者 此外， 免疫系统将通过进行植入， 免疫抑制受体，即，感染幼虫的蜗牛 吸虫 最后，初步介绍了 将获得异种移植物上被识别为非自身的决定簇 通过对移植物进行化学修饰 消化和凝集素结合。 该项目的潜在意义在于，它可能有助于 阐明免疫样自我识别的机制， 软体动物，作为研究自我的原始形式的模型 识别，也发生在脊椎动物，并提供信息 在一个重要的群体中， 软体动物

When tissue gafts are transplanted from one species of mollusc to another (xenografts), the grafts reportedly fail, i.e., die. The goal of the proposed research is to understand the mechanisms of xenograft failure in molluscs. Specifically, the study will assess the relative roles of (1) physiological incompatibility between donor and recipient and (2) immunological rejection by the recipient. Methods will include implantation of hearts from phylogenetically close and phylogenetically distant species into a single recipient snail species. Fates of the implants will be followed histologically and histochemically, and will be compared to fates of hearts maintained in vitro, and to fates of implants from donors of the same species. In addition, the role of the immune system will be ascertained by conducting implants in immunosuppressed recipients, i.e., snails infected with larval trematodes. Finally, initial information on the types of determinants on the xenograft recognized as non-self will be gained by subjecting grafts to chemical modification such as neuraminidase digestion and lectin binding prior to implantation. The potential significance of this project is that it may help to elucidate the mechanisms of immune-like self recognition in molluscs, serve as a model for studying primitive forms of self recognition that also occur in vertebrates, and provide information on phylogenetic relationships within an important group of molluscs.