Acquisition of a High Pressure Rapid Freezer

将使用高压快速冷冻机（HPRF）进行冷冻 相对较大尺寸的样品，而不形成 大冰晶，使研究复杂的细胞 系统，组织，使用快速冷冻固定。 这将打开新的 探索快速固定真核生物的新领域 有机体 再加上冷冻替代，快速冷冻将 允许研究不稳定的结构，短暂的事件和其他 与传统制剂相比， 已经使用这种方法研究了细胞悬浮液。 项目 将用冷冻的上级特性代替 用HPRF制备的组织，以研究短寿命事件 （由于离子浓度、分泌 在蜱的唾液腺中，植物中的钙分泌 细胞），不稳定结构（卵母细胞细胞骨架，棉纤维 细胞骨架），对磷敏感的结构（线粒体， 化疗，共生界面），免疫定位 各种抗原（细胞骨架、细胞分裂素、抗癌剂），和 甲藻与海葵、植物共生 和固氮细菌。 该项目涉及研究 几个区域机构，并将研究植物，动物， 和微生物系统， 使用先前可用的设备进行冷冻保存。 的 仪器将是唯一一个在东南部的联合国 将提供给合格的区域用户。

The High Pressure Rapid Freezer (HPRF) will be used to freeze samples of relatively large dimensions without the formation of large ice crystals, making it feasible to study complex cellular systems, tissues, using rapid cryofixation. This opens new frontiers in the exploration of rapidly fixed eukaryotic organisms. Coupled with freeze substitution, rapid freezing will allow study of labile structures, short lived events, and other advances over conventional preparations already shown when single cell suspensions have been studied using this approach. Projects will use the superior characteristics of freeze substituted tissues prepared with the HPRF to investigate short lived events (neural tissue alterations due to ion concentrations, secretion in salivary glands of ticks, secretion of calcium in plant cells), labile structures (oocyte cytoskeleton, cotton fiber cytoskeleton), osmotic-sensitive structures (mitochondria in chemotherapy, symbiotic interfaces), immunolocalization of various antigens (cytskeleton, cytokinin, anticancer agents), and symbiology between dinoflagellates and sea anemone, and plants and nitrogen-fixing bacteria. The project involves research in several regional institutions, and will examine plant, animal, and microbial systems that have been recalcitrant to cryopreservation using previously available equipment. The instrument will be the only one in the southeastern United States, and would be made available to qualified regional users.