H+-ATPase Subunits as Phylogenetic Markers

转运ATPase在所有活细胞的生物能量学中起着核心作用。在过去，我们已经证明了F-(真细菌)和V型(真核和考古细菌)ATPase对于早期生物进化是非常有用的分子标记。这项拟议的研究旨在将核编码的ATPase亚基的用途扩展到真核生物(特别是鞭毛虫和藻类)的早期辐射和陆地植物的进化。编码V型ATPase催化亚单位的部分基因将通过聚合酶链式反应(PCR)从各种生物的基因组或cDNA中直接扩增出来。获得的序列将用于确定这些生物之间的系统发育关系，包括评估派生的分支模式的重要性。

Transport ATPases play a central role in the bioenergetics of all living cells. In the past we have shown that the F-(eubacterial) and V-type (eukaryotic and archaebacterial) ATPases are very useful molecular markers for early organismal evolution. The proposed research aims to extend the use of the nuclear encoded ATPAse subunits to the early radiation of eukaryotes (especially flagellates and algae) and to the evolution of land plants. Parts of the genes encoding the catalytic subunits of V-type ATPASes will be amplified directly from the genomic or cDNA of various organisms using the polymerase chain reaction (PCR). The obtained sequences will be used to determine the phylogenetic relations between these organisms, including evaluations of the significance of the derived branching patterns.