Periplasmic Cyclic Adenosine Monophosphate Phosphodiesterasefrom Vibrio fischeri

费氏弧菌是一种海洋细菌，在单中心鱼的光器官中形成共生关系。最近对PI的发现表明，该细菌产生一种环状AMP的磷酸二酯酶(CDPase)，该酶具有不寻常的活性和特异性，并且位于周质中。为了评估这些共生菌与宿主之间基于核苷酸的生化相互作用的可能性，将通过广泛的分析方法，包括基因克隆和替换方法，来研究这种活性的生化特征、细胞调节和功能作用。

Vibrio fischeri is a marine bacterium that forms a symbiotic relationship in the light organs of monocentrid fish. A recent finding of the PI reveals that the bacterium produces a phosphodiesterase (CDPase) for cyclic AMP that is of unusual activity and specificity, and is periplasmic in location. The biochemical characteristics, cellular regulation, and functional role of this activity will be examined by a wide spectrum of analytical approaches, including gene cloning and replacement methods, in order to assess the possibility of nucleotide-based biochemical interactions between these symbiotic bacteria and their hosts.//