Structures of Complex Polysaccharides

提出了用核磁共振波谱、高效液相色谱和质谱学等物理方法测定具有生物化学意义的复杂碳水化合物的共价结构的研究。在粘蛋白糖蛋白的寡糖结构和复杂的细菌多糖中也提出了几个具体的实验。对利用完整糖蛋白的核磁共振波谱来探索和筛选粘蛋白中的碳水化合物结构的前景进行了评估。将使用~1H核磁共振和异核~1H检测~(13)C核磁共振波谱。创伤弧菌致病菌株的荚膜多糖的完整共价结构将被确定。完整的~1H和~(13)C核磁共振谱将被指认，并将确定酮基脱氧辛酸(KDO)残基的异常构型。展望了异核核磁共振波谱在脂多糖结构测定和构象研究中的应用前景。圆二色法测定复合碳水化合物糖基绝对构型的方法将扩展到新类型的单糖残基和发色多糖。将探索富含13C的细菌多糖用于异核核磁共振波谱，特别是三维核磁共振方法。

Research is proposed in the determination of the covalent structure of biochemically interesting complex carbohydrates by physical methods including NMR spectroscopy, high performance liquid chromatography and mass spectrometry. Several specific experiments are proposed in the structure of oligosaccharides from mucin glycoproteins and also in complex bacterial polysaccharides. The prospect of using NMR spectroscopy of intact glycoproteins for exploring and screening carbohydrate structure in mucins will be evaluated. 1H NMR and heteronuclear 1H detected 13C NMR spectroscopy will be used. The complete covalent structure of the capsular polysaccharide of a pathogenic strain of Vibrio vulnificus will be determined. The complete 1H and 13C NMR spectra of the Re-LPS (lipid A) from E. coli will be assigned and the anomeric configuration of the ketodeoxy octanoic acid (KDO) residues will be determined. Prospects for use of heteronuclear NMR spectroscopy for structure determination of lipopolysaccharides and exploration of the conformation will be evaluated. The circular dichroism method for determination of the absolute configuration of the sugar residues of complex carbohydrates will be extended to new types of monosaccharide residues and chromophoric polysaccharides. The use of 13C-enriched bacterial polysaccharides for heteronuclear NMR spectroscopy, especially 3-dimensional NMR methods will be explored.