Preparation of novel durable gene expression system comprising biodegradable device containing DNA complex particles coated by acidic polysaccharides

在这项研究中，我们使用可注射的自动形成的海藻酸凝胶制备了持久的基因表达系统，其中包括包被透明质酸的DNA复合体。当将包覆有透明质酸的微分散DNA复合体的藻酸盐溶液注射到活体中时，注射部位会形成凝胶。这种凝胶在肿瘤组织中不断降解。DNA复合体的凝胶降解和释放速率可以通过添加无定形磷酸钙(ACP)来控制。将含有细胞因子基因与ACP的DNA复合体的藻酸盐溶液靠近小鼠肿瘤组织注射时，显示出较高的治疗效果。它有望作为一种可注射的持续基因表达装置应用。

In this study, we prepared the durable gene expression systems using injectable auto-forming alginate gel including DNA complexes coated by hyaluronic acid. When the alginate solution including the finely dispersed DNA complexes coated with hyaluronic acid was injected in a living body, a gel was formed in the injected site. The gel continuously degraded in a tumor tissue. The rate of gel-degradation and release of the DNA complexes could be controlled by addition of amorphous calcium phosphate (ACP). When the alginate solution containing the DNA complexes with cytokine-gene and ACP was injected close to the tumor tissue of the mice, the formulation showed high therapeutic effect. It is expected for applications as an injectable sustained-gene expression device.