Preparation of novel durable gene expression system comprising biodegradable device containing DNA complex particles coated by acidic polysaccharides

在这项研究中，我们使用可注射的自形成海藻酸凝胶制备了持久的基因表达系统，该凝胶包括透明质酸包被的DNA复合物。当将海藻酸盐溶液注入活体时，其中包含了透明质酸包裹的精细分散的DNA复合物，在注射部位形成凝胶。凝胶在肿瘤组织中不断降解。加入无定形磷酸钙（ACP）可以控制凝胶降解和DNA复合物释放的速度。将含有细胞因子基因和ACP DNA复合物的藻酸盐溶液在小鼠肿瘤组织附近注射，显示出较高的治疗效果。它有望应用于可注射的持续基因表达装置。

In this study, we prepared the durable gene expression systems using injectable auto-forming alginate gel including DNA complexes coated by hyaluronic acid. When the alginate solution including the finely dispersed DNA complexes coated with hyaluronic acid was injected in a living body, a gel was formed in the injected site. The gel continuously degraded in a tumor tissue. The rate of gel-degradation and release of the DNA complexes could be controlled by addition of amorphous calcium phosphate (ACP). When the alginate solution containing the DNA complexes with cytokine-gene and ACP was injected close to the tumor tissue of the mice, the formulation showed high therapeutic effect. It is expected for applications as an injectable sustained-gene expression device.