Mechanism of Genomic Reprogramming at the Onset of Embryogeny

植物基因组的可塑性允许组织改变 从一种形式发展到另一种形式。 一旦发展 命运是确定的，一系列的形态变化的结果， 伴随着基因表达的连续变化。 的 发育开关的机制知之甚少， 除了可能发生主要的基因组重编程。 最近， PI发现晚期胚胎发生丰富（莱亚）基因 从受精卵到胚胎发育开始时， 培养的小孢子和培养的叶组织。 这一结果 表明胚胎期的转换涉及到一个全面的去压抑 或激活一系列基因，包括早期和晚期 胚胎基因 为了证实这一假设，PI 建议研究基因表达的机制， 胚胎发生的过程 实验旨在比较 莱亚基因DC8在早期表达的机制， 胚胎发育后期，并确定参与的基因， DC8启动子的激活。

The plasticity of the plant genome permits the tissue to change from one form of development to another. Once the developmental fate is determined, a series of morphological changes result that are accompanied by sequential changes in gene expression. The mechanism of the developmental switch is poorly understood, except that major genomic reprogramming might occur. Recently, the PI found that late embryogenesis abundant (LEA) genes are activated at the onset of embryogeny from the zygote, from cultured microspores and from cultured leaf tissue. This result suggests that the embryonic switch involves a global derepression or activation of a battery of genes including both early and late embryonic genes. To substantiate this hypothesis, the PI proposes to study the mechanisms of gene expression at the onset of embryogenesis. Experiments are designed to compare the mechanisms by which a LEA gene, DC8, is expressed at early and late embryogeny, and to identify the genes involved in the activation of the DC8 promotor.