Large Scale Displacement High Performance Liquid Chromatography for Separation of Peptides and Proteins

这个研究项目的目标 是开发一个系统， 高效、经济、高纯度 蛋白质和肽的回收 通过置换色谱法。 在 为了满足纯度 人类治疗的要求 生物技术产业 采用最先进的分离技术 从蛋白质中借用的技术 化学. 最高之列 分辨率分离过程是 高效液相 色谱法（HPLC），其具有 在一个大的 用于蛋白质纯化的规模。 阻碍更广泛发展的主要因素 工艺规模HPLC的验收 纯化，除了 柱和设备的高成本， 溶剂体积大， 柱容量和损失 生物活性，特别是 蛋白质不可逆地 变性或聚集在 浓度，并根据 期间遇到的条件 典型的色谱操作。 置换色谱法是一种 另一种产量高得多的方法 吞吐量，更高效 利用固定资产， 移动的相位和高分辨率。 此外，位移 色谱法可以在 常规的离子交换和 反相色谱 列，不需要 昂贵的生产外来 亲和柱试剂 分离技术，它 包括操作步骤， 容易放大。

The goal of this research program is to develop systems for efficient, economical, high-purity recovery of proteins and peptides by displacement chromatography. In order to meet the purity requirements for human therapeutic use, the biotechnology industry employs state-of-the-art separation techniques borrowed from protein chemistry. Among the highest resolution separation processes is high performance liquid chromatography (HPLC), which has found a limited use on a large scale for purification of proteins. The main features impeding broader acceptance of process scale HPLC purification, in addition to the high cost of columns and equipment, are the large solvent volumes, low column capacity and loss of biological activity, particularly for proteins that irreversibly denature or aggregate at the concentrations, and under the conditions encountered during typical chromatographic operations. Displacement chromatography is an alternative to yield much higher throughput, more efficient utilization of stationary and mobile phases, and high resolution. In addition, displacement chromatography can be performed on conventional ion exchange and reversed phase chromatography columns, does not require the expensive production of exotic column reagents as do affinity separation techniques, and it involves operating steps that are readily scaled up.