U.S.-Hungary Joint Fund Research on Microspores for Maize Transformation

纽约州立大学奥尔巴尼分校约瑟夫·马斯卡伦哈斯博士和比亚塔·巴纳巴斯博士在匈牙利联合基金下开展的这一美国-匈牙利研究项目的主要目标是，匈牙利科学院农业研究所将开发一种方法，从花药培养的小孢子中生产成熟的、可存活的花粉。这项工作将包括在微弹轰击之前通过在花药壁上切开来暴露小孢子，然后花粉将在花药中成熟。如果这项初步工作表明该系统似乎运行良好，在花药中成熟的花粉将用于授粉，以研究玉米植株的稳定转化。玉米在东欧和美国都是一种非常重要的农作物，利用花粉进行简单的转化程序将对遗传学和发育生物学的基础研究具有重要意义并在工程中培育出具有农艺价值的新性状。这个植物生理学项目通过使美国和东欧的顶尖专家能够结合互补的人才和集合研究来实现推进科学知识的计划目标在双方都感兴趣和有能力的领域提供资源。

The primary objective of this U.S.-Hungary research project under the Hungary Joint Fund between Dr. Joseph Mascarenhas, State University of New York at Albany and Dr. Beata Barnabas, Agricultural Research Institute of the Hungarian Academy of Sciences, is to develop methods for the production of mature, viable pollen from microspores cultured in anthero. The work will involve exposing microspores by making a cut in the anther wall prior to micro- projectile bombardment and the pollen will then be matured in anthero. If and when this preliminary work indicated that the system seems to be working well, the pollen matured in anthero will be used for pollination to study stable transformation of maize plants. Maize is a very important agricultural crop both in Eastern Europe and the US and a simple transformation procedure using pollen would be of significance for basic studies in genetics and developmental biology of the plant and in the engineering of new traits of agronomic value. This project in plant physiology fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents and pool research resources in areas of strong mutual interest and competence.