Structure, Energetics and Reaction Dynamics of Bimolecules in the Gas Phase

Beauchamp教授获得了实验物理化学项目的资助，研究生物分子在气相中的性质、结构和反应动力学。长期目标是为亚皮摩尔样品的氨基酸和核苷酸序列测定设计快速可靠的方法。软电离技术，包括激光解吸，快速原子轰击和电喷雾电离将被用于形成热脆弱生物分子的单和多带电分子离子。表征的主要技术是傅里叶变换离子回旋共振光谱。测定蛋白质中的氨基酸序列和核苷酸中的碱基对只需要少量的样品，将使用从这些热脆弱的生物分子中轻轻去除电子的技术，然后捕获、操纵和检测已经形成的离子。

Professor Beauchamp is supported by a grant from the Experimental Physical Chemistry Program to investigate the properties, structures, and reaction dynamics of biomolecules in the gas phase. The long term objective is to devise rapid and reliable methods for amino acid and nucleotide sequence determinations with subpicomole samples. Soft ionization techniques, including laser desorption, fast atom bombardment, and electrospray ionization will be used to form singly and multiply charged molecular ions of thermally fragile biomolecules. The major technique for characterization is Fourier transform ion cyclotron resonance spectroscopy. %%% The determination of the sequence of amino acids in proteins and of the base pairs in nucleotides requiring only minute samples will be carried out using techniques which gently remove electrons from these thermally fragile biomolecules, and then trap, manipulate and detect the ions which have been formed.