Fundamental Studies of the Mechanisms of Desolvated Ion Formation from Small Charged Droplets

带电小液滴形成去溶剂化离子机制的基础研究

• 批准号: 9727566
• 负责人: Jesse Beauchamp
• 金额: $ 31.5万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 2002-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9727566&HistoricalAwards=false
• 关键词: Fundamental; Studies; Mechanisms; Desolvated; Ion

This research project, supported in the Analytical and Surface Chemistry Program, addresses the dynamics and temporal evolution of highly charged micron sized droplets. The goal of the work being carried out by Professor Beauchamp at CalTech is to determine the mechanism of formation of desolvated ions, which provides the fundamental basis of ion formation in electrospray ion sources used in mass spectrometry. Electrospray ionization is very useful for the mass spectrometric analysis of large biologically significant molecules. The ionization mechanism is probed by the use of ion mobility spectrometry coupled with fluorescence probing of dye tagged droplets. %%% In order to determine the mechanism of solvent loss and ion formation in the electrospray ionization source mass spectrometer, this research project is investigating single droplet ion mobility. Mobility measurements coupled with laser induced fluorescence tagging of droplets will provide time dependent information on the ion desolvation process. An increased understanding of the ion formation mechanism will improve the applicability of mass spectrometry to the structural analysis of large, biologically significant compounds.

本研究项目，在分析和表面化学支持 程序，解决了动力学和时间演变的高度带电 微米大小的液滴。 正在开展的工作的目标是 加州理工学院的比彻姆教授要确定 去溶剂化的离子，这提供了离子形成的基本基础 在质谱法中使用的电喷雾离子源中。 电喷雾 电离对于大分子的质谱分析非常有用。 具有生物学意义的分子。 探讨了离子化机理 通过使用与荧光探测耦合的离子迁移谱法 染料标记的液滴。 %为了确定中溶剂损失和离子形成的机制 电喷雾电离源质谱仪，本研究 该项目正在研究单液滴离子迁移率。 移动性 与液滴的激光诱导荧光标记相结合的测量 将提供关于离子去溶剂化过程的时间依赖性信息。 增加对离子形成机制的理解将提高 质谱法对结构分析的适用性 大的，有生物学意义的化合物。