Insertion Mutagenesis in Arabidopsis: Toward a Standard Population

费尔德曼博士将使用T-DNA标记方法通过种子感染程序生产独特的拟南芥转化体。目标是每年生产5000条生产线。转化体将首先在琼脂平板上筛选，然后在土壤种植的植物中筛选。所有在表型上有明显改变的品系都将被记录下来，并提供给一般科学界。种子的供应情况将通过拟南芥电子公告板公布。希望筛选特定表型的科学家将被允许来亚利桑那大学逐个进行筛选。费尔德曼博士计划在这一资助期结束时将突变株存放在国家拟南芥资源中心。种子转化在拟南芥基因标记和克隆中的重要性已经确立。该项目将为植物分子生物学研究提供宝贵的资源。

Dr. Feldmann will produce unique transformants of Arabidopsis with the seed infection procedure using the T-DNA tagging method. The target is to produce 5,000 lines a year. The transformants will be screened initially on agar plates, and then in soil grown plants. All lines that have a visible alteration in phenotype will be recorded and made available to the general scientific community. The availability of the seeds will be announced through the electronic Arabidopsis bulletin board. Scientists who wish to screen for specific phenotypes will be permitted to come to University of Arizona to do so on a case by case basis. Dr. Feldmann plans to deposit the mutant lines to a national Arabidopsis resource center at the end of this grant period. The importance of seed transformation in the tagging and cloning of Arabidopsis genes have been established. It is extremely time- consuming to produce the tagged lines and this project will provide invaluable resources to the plant molecular biology community.