Multi-User Triple Quadrupole Electrospray Mass Spectrometer

该提案要求提供资金，用于购买具有在线高效液相色谱和串联质谱学能力的电喷雾三重四极杆质谱仪。按照计划，该仪器将主要由W·奥尔顿·琼斯细胞科学中心的9个用户组提供服务。一系列独立支持的研究正在进行中，所有这些研究都需要所要求的仪器。大多数项目是针对多肽和蛋白质的分析，拟议的仪器将设在机构共享蛋白质化学设施内并在其内部运作。该仪器将首次为用户群体提供对覆盖相对较宽质量范围的多肽和蛋白质进行低到亚皮克摩尔水平的准确质谱分析的便利条件。细胞科学中心目前没有质谱仪。质谱学已经成为多肽和蛋白质结构功能研究不可或缺的工具，对于W.Alton Jones细胞科学中心正在进行的和未来的生物学研究取得令人满意的进展至关重要。

This proposal requests funds for the purchase of an electrospray triple-quadrupole mass spectrometer with on line HPLC and tandem mass spectrometry capability. It is planned that the instrument will serve primarily by nine user groups at the W. Alton Jones Cell Science Center. A broad range of independently supported studies are ongoing and all require the requested instrumentation. Most of the projects are directed at analysis of peptides and proteins and the proposed instrument will be located and operated within the institutional share Protein Chemistry Facility. The instrument will provide the user groups with ready access for the first time to low to sub-picomole level accurate mass spectrometric analyses of peptides and proteins covering a relatively broad mass range. There currently are no mass spectrometry instruments at the Cell Science Center. Mass spectrometry has become an indispensable tool for peptide and protein structure- function studies and is essential for satisfactory progress in the ongoing and future biological research at the W. Alton Jones Cell Science Center.