Control of Cell Specification and Gene Expression in Anther Development

开花植物的雄性生殖过程发生在 花药 这个器官是由几个专门的组织组成的 和细胞类型，并负责产生花粉粒 含有精子细胞的组织 花药组织有序形成 花内细胞谱系的模式 分生组织 这些组织从前体细胞分化而来， 出现在特定的位置，或领土，在 发育中的花药原基。 的分子过程 负责不同花药细胞类型的特化 在原基起始后的变化是未知的。 也不明白 基因组是如何在特定的花药细胞类型中表达的 在花药发育过程中被协调激活。 本提案中概述的实验将利用嵌合 细胞毒性和抗细胞毒性基因，以解决的作用 花药发育过程中细胞间的相互作用 细胞类型。 其他实验将利用分子策略 确定必要的调节电路元件， 激活一个花药组织内的几个非同源基因， 绒毡层 这些实验的意义在于， 应该为沟通过程提供新的见解 花药细胞类型分化所需的，提供 进入分化所需的进程， 花药细胞类型，提供进入分子途径 负责这些细胞类型的规格，以及 产生关于机制的新的概念信息， 在植物发育过程中控制细胞特异性基因表达。

Male reproductive processes in flowering plants occur in the anther. This organ is composed of several specialized tissues and cell types and is responsible for producing pollen grains that contain the sperm cells. Anther tissues form in an orderly pattern from cell lineages that are present within the floral meristem. These tissues differentiate from precursor cells that appear at specific locations, or territories, within the developing anther primordium. The molecular processes responsible for the specification of different anther cell types after primordium initiation are not known. Nor is it understood how gene sets that are expressed in specific anther cell types are activated coordinately during anther development. Experiments outlined in this proposal will utilize chimeric cytotoxic and anticytotoxic genes to address the role of cell/cell interaction processes in the specification of anther cell types. Other experiments will utilize molecular strategies to identify elements of the regulatory circuitry necessary to activate several non-homologous genes within one anther tissue, the tapetum. The significance of these experiments is that they should provide new insight into the communication processes required for the differentiation of anther cell types, provide entry into the processes required for the differentiation of anther cell types, provide entry into the molecular pathways responsible for the specification of these cell types, and generate new conceptual information on the mechanisms that control cell-specific gene expression during plant development.