Male germline regulatory mechanisms integrating cell cycle control with gamete specification.

雄性种系调节机制将细胞周期控制与配子规范相结合。

基本信息

  • 批准号:
    BB/I011269/1
  • 负责人:
  • 金额:
    $ 49.26万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2011
  • 资助国家:
    英国
  • 起止时间:
    2011 至 无数据
  • 项目状态:
    已结题

项目摘要

Plant sexual reproduction is of vital importance both in the natural environment and for crop production. As in animals, plant reproduction relies on the production of male and female reproductive cells (sperms and eggs) that are united at fertilization. This research aims to uncover the underlying processes that control the production and fertility of plant sperm cells that are formed within pollen grains during sexual reproduction. Surprisingly, the molecular pathways that regulate sperm cell formation in flowering plants are largely unknown, even though this process is vital for plant breeding, crop and seed production. We have identified a master regulator gene, termed DUO1 that is vital for sperm cell formation and fertility in the model plant species Arabidopsis thaliana, a close relative of important seed and vegetable crops in the mustard family. The DUO1 gene has an important coordinating role in plant sperm cell development, controlling the activity of a wide network of genes involved in the control of cell division, and in the specialised function of sperm cells at fertilization. In the proposed work we will investigate for the first time how this network of regulatory factors controls plant sperm cell production using a range of technologies involving molecular, genetic and cell biological approaches. We will identify how different sets of genes are coordinated within this network to control cell division and the specialised production of plant sperm cells. This project will provide valuable information on how plants reproduce, allow meaningful comparisons to animal reproduction and generate new knowledge and technology that will allow greater understanding of this economically important part of the plant life cycle. Moreover, collaborative work with rice reproductive biology experts working in China has been established providing a direct route to evaluate the transfer of knowledge in an important food crop in which technologies such as hybrid seed production have significant (~20%) yield advantage. The work will be carried out by a full-time postdoctoral research assistant and a laboratory technician at the University of Leicester. Some aspects of the work will involve collaboration with researchers involving exchange of data and materials with Universities in China and Japan. Some of the specialised high throughput molecular work will be carried out using services provided by the VIB research institute, Belgium.
植物的有性生殖在自然环境和作物生产中都具有重要意义。与动物一样,植物的繁殖依赖于受精时结合的雄性和雌性生殖细胞(精子和卵子)的产生。这项研究旨在揭示控制植物精细胞产生和生育力的潜在过程,这些精细胞在有性生殖过程中在花粉粒内形成。令人惊讶的是,在开花植物中调节精细胞形成的分子途径在很大程度上是未知的,尽管这个过程对植物育种,作物和种子生产至关重要。我们已经确定了一个主调节基因,称为DUO1,这是至关重要的精子细胞的形成和生育力的模式植物物种拟南芥,一个重要的种子和蔬菜作物在芥菜家族的近亲。DUO1基因在植物精细胞发育中具有重要的协调作用,控制参与细胞分裂控制的广泛基因网络的活性,以及受精时精细胞的专门功能。在拟议的工作中,我们将首次研究这种调控因子网络如何使用一系列涉及分子,遗传和细胞生物学方法的技术来控制植物精细胞的生产。我们将确定不同的基因组如何在这个网络中协调控制细胞分裂和植物精细胞的专门生产。该项目将提供有关植物如何繁殖的宝贵信息,允许与动物繁殖进行有意义的比较,并产生新的知识和技术,使人们能够更好地了解植物生命周期中这一经济上重要的部分。此外,还与在中国工作的水稻生殖生物学专家开展了合作,为评估一种重要粮食作物的知识转让提供了一条直接途径,在这种作物中,杂交种子生产等技术具有显著的产量优势(约20%)。这项工作将由莱斯特大学的一名全职博士后研究助理和一名实验室技术员进行。这项工作的某些方面将涉及与研究人员的合作,包括与中国和日本的大学交换数据和材料。一些专门的高通量分子工作将使用比利时VIB研究所提供的服务进行。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Genetic control of generative cell shape by DUO1 in Arabidopsis.
  • DOI:
    10.1007/s00497-023-00462-x
  • 发表时间:
    2023-09
  • 期刊:
  • 影响因子:
    3.4
  • 作者:
  • 通讯作者:
Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants.
  • DOI:
    10.1007/978-1-4939-7286-9_22
  • 发表时间:
    2017
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Benjamin Peters;Jack Aidley;M. Cadzow;D. Twell;L. Brownfield
  • 通讯作者:
    Benjamin Peters;Jack Aidley;M. Cadzow;D. Twell;L. Brownfield
Epigenetic reprogramming rewires transcription during the alternation of generations in Arabidopsis.
  • DOI:
    10.7554/elife.61894
  • 发表时间:
    2021-01-25
  • 期刊:
  • 影响因子:
    7.7
  • 作者:
    Borg M;Papareddy RK;Dombey R;Axelsson E;Nodine MD;Twell D;Berger F
  • 通讯作者:
    Berger F
Overexpression of TWO-IN-ONE Domains Inhibits Cytokinesis in Arabidopsis
二合一结构域的过度表达抑制拟南芥中的细胞分裂
  • DOI:
    10.1007/s12374-022-09353-6
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    2.9
  • 作者:
    Oh S
  • 通讯作者:
    Oh S
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David Twell其他文献

AtCSLA 7 , a Cellulose Synthase-Like Putative Glycosyltransferase , Is Important for Pollen Tube Growth and Embryogenesis in Arabidopsis 1
AtCSLA 7 是一种类似纤维素合酶的假定糖基转移酶,对拟南芥花粉管生长和胚胎发生很重要 1
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    F. Goubet;Audrey Misrahi;S. Park;Zhinong Zhang;David Twell;P. Dupree
  • 通讯作者:
    P. Dupree
halfman, an Arabidopsis male gametophytic mutant associated with a 150 kb chromosomal deletion adjacent to an introduced Ds transposable element
  • DOI:
    10.1007/s00497-003-0181-9
  • 发表时间:
    2003-07-04
  • 期刊:
  • 影响因子:
    2.400
  • 作者:
    Sung-Aeong Oh;Soon Ki Park;Inok Jang;Ross Howden;James M. Moore;Ueli Grossniklaus;David Twell
  • 通讯作者:
    David Twell
Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree)
  • DOI:
    10.1007/bf00034939
  • 发表时间:
    1986-01-01
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Gert Ooms;David Twell;Margreet E. Bossen;J. Harry C. Hoge;Michael M. Burrell
  • 通讯作者:
    Michael M. Burrell
Expression of shoot-inducing Ti TL-DNA in differentiated tissues of potato (Solanum tuberosum cv Maris Bard)
马铃薯 (Solanum tuberosum cv Maris Bard) 分化组织中芽诱导 Ti TL-DNA 的表达
  • DOI:
  • 发表时间:
    1985
  • 期刊:
  • 影响因子:
    5.1
  • 作者:
    Michael M. Burrell;David Twell;Angela Karp;G. Ooms
  • 通讯作者:
    G. Ooms
Correction: Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis
  • DOI:
    10.1007/s00497-023-00471-w
  • 发表时间:
    2023-06-15
  • 期刊:
  • 影响因子:
    2.400
  • 作者:
    Božena Klodová;David Potěšil;Lenka Steinbachová;Christos Michailidis;Ann-Cathrin Lindner;Dieter Hackenberg;Jörg D. Becker;Zbyněk Zdráhal;David Twell;David Honys
  • 通讯作者:
    David Honys

David Twell的其他文献

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{{ truncateString('David Twell', 18)}}的其他基金

14 ERA-CAPS. EVOREPRO. Evolution of Sexual Reproduction in Plants
14 个 ERA-CAPS。
  • 批准号:
    BB/N005090/1
  • 财政年份:
    2015
  • 资助金额:
    $ 49.26万
  • 项目类别:
    Research Grant
Engineering reversible male sterility - improved methods for hybrid seed production
工程可逆雄性不育——改进杂交种子生产方法
  • 批准号:
    BB/M00502X/1
  • 财政年份:
    2014
  • 资助金额:
    $ 49.26万
  • 项目类别:
    Research Grant
Epigenetic control of the plant male gametophyte and germline
植物雄配子体和种系的表观遗传控制
  • 批准号:
    BB/F007558/1
  • 财政年份:
    2008
  • 资助金额:
    $ 49.26万
  • 项目类别:
    Research Grant
Cellular and Molecular Mechanisms of Cytokinesis Mediated by the TIO Fused Kinase
TIO 融合激酶介导的细胞分裂的细胞和分子机制
  • 批准号:
    BB/E001017/1
  • 财政年份:
    2007
  • 资助金额:
    $ 49.26万
  • 项目类别:
    Research Grant

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原生动物四膜虫生殖小核(germline nucleus)体功能(somatic function)的分子基础研究
  • 批准号:
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