Development of an Optical Laser Trap for the Study of Biological Media and Cellular Interactions (Resubmission of DIR-9102304)

光学激光捕获技术是一种潜在的强大的 研究光与生物的基本相互作用的工具 在微观尺度上的媒介。 我们有能力去分析，量化， 并预测相干辐射力的影响将是关键 有效利用和发展其作为一种分析工具， 生物医学和外科应用。 在此，我们描述了 开发单光束红外光学捕获系统，以及 其作为显微操作工具和非接触力用途 传感器，在生物介质和细胞的研究 该系统由可调谐激光源、 高分辨率微力传感器，前向散射检测器， 和数据采集系统，是用于（1） 捕获产生的力和热贡献的量化 限制过程;和（2）红细胞的基础研究， 染色体和细胞器显微操作行为在一个单一的 束光势 其他潜在应用 还描述了本文要开发的光学捕获系统。 人工工程有机脂质体模型被呈现为 一种以受控的方式确定物理和 细胞和细胞膜样品的光学特性，以及 它们对光阱性能的影响。 此外，一盏灯 散射模型，和一套实验测量，是要 用于理论和实验结果的相关性。 在一种情况下，这种定量理论被应用于细胞， 沿着微管运输机制的生物学研究。 一 物理参数的工作知识， 用于产生和维持光阱，以及它们对 宿主生物样本，应该对研究人员和 光学捕获系统的从业者。

The technique of optical laser trapping is, potentially, a powerful tool for studying fundamental interactions of light with biological media on a microscopic scale. The ability to interprete, quantify, and predict the effects of coherent radiation forces, will be key to its effective use and development as an analytical tool in biomedical and surgical applications. Herein, we describe the development of a single beam, infrared optical trapping system, and its use, as a micromanipulation tool and a noncontact force transducer, in the study of biological media and cellular interactions, The system, consisting of a tunable laser source, a high resolution microforce transducer, forward scattering detector, and data acquisition system, is to be used in the (1) quantification of trap generated forces and thermal contributions to the confinement process; and (2) basic study of red blood cell, chromosome, and organelle micromanipulation behavior in a single beam optical potential. Other potential applications for the optical trapping system to be developed herein are also described. An artificially engineered organic liposome model is presented as a way of determining, in a controlled fashion, the physical and optical characteristics of cell and cell membrane samples, and their effect on optical trapping performance. In addition, a light scattering model, and set of experimental measurements, is to be used in the correlation of theoretical and experimental results. This quantitative theory is applied, in one case, to the cell biology study of transport mechanisms along microtubules. A working knowledge of the physical parameters that are responsible for generating and maintaining an optical trap, and their effect on host biological samples, should be useful to both researchers and practitioners of optical trapping systems.