Modulation of Two Transient Potassium Currents in Mouse Hippocampal Neurons

神经元的个体性是由特定的 存在于神经元中的功能性离子通道的补充 膜的 不同的神经元表达不同数量的通道 对钠、钙、钾和氯离子具有选择性， a consequence后果have specific特定patterns模式of responses反应to incoming传入 兴奋性和抑制性刺激。 为了理解 建立了神经元的个体性，一类钾 电流的活动影响两个波形的单一 动作电位和重复动作电位的模式 将被研究。 实验将在培养的 胚胎小鼠海马神经元， 早期神经发育研究。 之前已经观察到 两种不同形式的瞬时信号在神经元中的表达 钾电流，称为A电流和D电流，取决于 与非神经胶质细胞接触。 由于A电流和D电流 参与神经元电发生，这些观察结果提高了 神经胶质细胞通过调节神经元活动的可能性 影响渠道表达。 为了了解这个过程 可能会发生，A-和A-之间的精确分子关系 D-电流通道应予以澄清。 在本实验中 在单通道处的门控和渗透行为的差异 水平将被检查。 未来的实验将侧重于以下问题： 基因表达和翻译后修饰， 确定A-1的离子通道之间的差异， D电流

The individuality of neurons is determined by the particular complement of functional ion channels present in the neuronal membrane. Different neurons express varying numbers of channels selective for sodium, calcium, potassium and chloride ions, and as a consequence have specific patterns of responses to incoming excitatory and inhibitory stimuli. In order to understand how the individuality of neurons is established, a class of potassium currents whose activity influences both the waveform of single action potentials and the pattern of repetitive action potentials will be studied. Experiments will be performed on cultured embryonic mouse hippocampal neurons, a standard preparation for study of early neural development. It has been previously observed that the expression in neurons of two variant forms of transient potassium currents, termed A-current and D-current, is dependent on contact with non-neuronal glial cells. Since A- and D-currents are involved in neuronal electrogenesis, these observations raise the possibility that glial cells modulate neuronal activity by influencing channel expression. To understand how this process might occur, the precise molecular relationship between A- and D-current channels should be clarified. In the present experiments differences in gating and permeation behavior at the single channel level will be examined. Future experiments will focus on issues of gene expression and post-translational modification that may establish the differences between the ion channels underlying A- and D-currents.