Long and Medium-Term Research: Isolation and Characterization of Inhibitors of FOS/JUN DNA-Binding Function

中长期研究：FOS/JUN dna结合功能抑制因子的分离与表征本奖项由国外卓越中心长期和中期研究项目资助。它使美国科学家和工程师能够在国外公认的卓越研究中心进行三到十二个月的研究。该计划的奖励为联合研究提供了机会，并利用独特或互补的设施、专业知识和国外的实验条件。该奖项将支持Carlos Molina-Nazario博士与Paolo Sassone- Corsi博士在法国斯特拉斯堡CNRS真核生物遗传分子实验室进行为期12个月的博士后研究访问。本研究项目的目的是寻找Fos/Jun反激活活性的抑制因子。Fos和Jun是转录激活因子，属于早期反应基因类。它们介导细胞外信号转化为长期的细胞反应，如细胞生长和分化。它们也被定义为原癌基因，因为如果被激活，它们可以作为转化基因。转化活动似乎与转化特性有关。因此，参与抑制Fos-Jun反激活特性的因素可能参与了细胞增殖和转化的控制。本课题的目的可以概括为：分离和表征Fos/Jun功能特异性抑制因子IP-1，研究其参与Fos/Jun反激活特性的调控，并表征IP-1在细胞增殖和转化中的作用。学习这些独特的分子生物学方法和分子癌变的一般系统将有助于Molina-Nazario博士对癌症发病的分子机制的未来研究。奖励建议提供的资金用于支付12个月的津贴、受抚养人津贴和往返机票。

Long & Medium-Term Research: Isolation and Characterization of Inhibitors of FOS/JUN DNA-Binding Function This award is under the Long and Medium-Term Research at Foreign Centers of Excellence Program, which enables U.S. scientists and engineers to conduct three to twelve months of research abroad at research centers of proven excellence. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. Carlos Molina-Nazario to work with Dr. Paolo Sassone- Corsi at the Laboratoire de Genetique Molecularie des Eucaryotes, CNRS in Strasbourg, France. The purpose of this research project is to find suppressor(s) of the Fos/Jun transactivating activity. Fos and Jun are transcriptional activators that belong to the early response gene class. They mediate the translation of extracellular signals into long term cellular response such as cell growth and differentiation. They are also defined proto-oncogenes because they can act as transforming genes if activated. The transforming activity seems to be related to the transactivating properties. Therefore factors involved in the suppression of the Fos-Jun transactivating properties are likely to be involved in the control of the cell proliferation and transformation. The purpose of the project can be summarized as follows: to isolate and characterize the factor IP-1 (a specific repressor of Fos/Jun function) and study its involvement in the control of Fos/Jun transactivating properties, and to characterize IP-1's role in cell proliferation and transformation. The learning of these unique methods in molecular biology and the general systems of molecular carcinogenesis will help Dr. Molina-Nazario for the future study of the molecular mechanism involved in the onset of cancer. The award recommendation provides funds to cover a stipend for twelve months, a dependent allowance and return airfare.