Molecular Characterization of Vacuolar Membrane, Calcium Binding Proteins

胞浆功能对于调节胞质内 钙水平。 液泡膜特有的钙结合蛋白 膜已被识别。 这个项目的近期目标 是从亲和性的角度来纯化和表征这些蛋白质， 容量和阳离子选择性。 多克隆抗体将是 用于研究这些组织特异性表达模式， 蛋白质，特别是关于空泡化过程 和液泡膜系统的生物发生。 抗体将 进一步用于鉴定表达文库中的cDNA克隆 编码这些蛋白质。 %%% 植物细胞含有大的膜封闭的隔间， 提供了许多生物化学和结构功能， 植物细胞 其中一些是相对“通用”的功能 通常与溶酶体型隔室相关;其他的是 对植物来说独一无二 因此，液泡在 在增长和发展。 值得注意的是， 膜活动已经检查，更少的仍然已经 在分子水平上进行鉴定和检测。 还没有 对这种细胞器的起源有一个清晰的了解， 液泡膜蛋白连续地 补充。 这项研究将有助于了解 液泡的分子基础， 高等植物对环境的反应能力， 正常的生长和发育过程。

Vacuolar function is essential to the regulation of cytosolic calcium levels. Calcium-binding proteins unique to the vacuolar membrane have been identified. The immediate goal of this project is to purify and characterize these proteins in terms of affinity, capacity, and cation selectivity. Polyclonal antibodies will be used to study the tissue-specific expression patterns of these proteins, particularly with regard to the process of vacuolation and biogenesis of the vacuole membrane system. Antibody will further serve to identify cDNA clones in expression libraries encoding these proteins. %%% Plant cells contain large membrane-enclosed compartments, which serve a number of biochemical and structural functions for the plant cell. Some of these are relatively "universal" functions associated generally with lysosome-type compartments; others are unique for plants. Accordingly, the vacuole has an essential role in growth and development. It is remarkable that few vacuolar membrane activities have been examined and fewer still have been identified and examined at the molecular level. There is not yet a clear understanding of either the origins of this organelle or the means by which vacuolar membrane proteins are continuously replenished. This study will contribute to an understanding of the molecular basis for the role of the vacuole in conferring to higher plants the ability to respond to its environment and carry out the normal processes of growth and development.