D-raf in Drosophila Signal Transduction

该项目的长期目标是确定 D-raf介导的信号转导在建立和 果蝇模式的阐述。具体 本项目的目标如下：1）测试模型， D-raf作为丝氨酸/苏氨酸激酶的活化依赖于 诱导“末端”信号转导途径，2） 表征D-raf蛋白在 发展，和3）确定基因编码的其他成员， D-raf介导的信号转导途径。因为D-raf是 原癌基因Raf-1的果蝇同源物， 其发展作用的特点和确定 它的调节机制将提供线索， 了解高等生物的信号转导。 在该提案中设计了实验来解决 以下问题。D-raf蛋白是否表现出 根据其同源性预测的丝氨酸/苏氨酸激酶活性 Raf-1？磷酸化和蛋白质的磷酸化 D-raf状态及其作为丝氨酸/苏氨酸的能力 激酶？在细胞的什么位置，在什么发育阶段， D-raf蛋白存在吗还有哪些类型的基因产物 在D-raf介导的信号转导通路中的作用这些问题 将用细胞生物学、遗传学和分子生物学来回答 手段

The long term objective of this project is to define the role of D-raf mediated signal transduction in the establishment and elaboration of pattern in Drosophila melanogaster. The specific aims of this project are as follows: 1) to test the model that activation of D-raf as a serine/threonine kinase depends upon induction of the "terminal" signal transduction pathway, 2) to characterize the pattern of D-raf protein distribution in development, and 3) to identify genes encoding other members of D-raf mediated signal transduction pathways. Since D-raf is the Drosophila homologue of the Raf-1 proto-oncogene, the characterization of its developmental role and the determination of its mechanisms(s) of regulation will provide clues toward understanding signal transduction in higher organisms. Experiments have been designed in this proposal to address the following questions. Does the D-raf protein display a serine/threonine kinase activity as predicted by its homology with Raf-1? Is there a relationship between the phosphorylation state of D-raf and its ability to act as a serine/threonine kinase? Where in the cell, and at what developmental stages is the D-raf protein present? What other types of gene products act in D-raf mediated signal transduction pathways? These questions will be answered using cell biological, genetic and molecular means.