Population Genetics of Transposable Elements

该项目将继续调查人口 果蝇属转座因子的性质。 问题 是否某些元素在某些地方的明显固定 染色体位点。黑腹果蝇种群是真实的， 人工智能将得到解答。 D. melanogaster 在以前的研究中使用的元素将被表征为 限制性酶切作图 这将产生重要的信息， 对以往工作的解释，以及其他建议 实验 将对转座率进行研究 并切除了D.黑腹素 Southern印迹和原位杂交的元素探针将 用于检测等基因原种中元素位置的变化 保持杂合状态， 还将研究超过100代的重组。 D.转座因子家族美国将被克隆。 这些将用于确定元素是否已累积 和理论上预期的一样，这个物种的新Y染色体。 拟议的实验将提供信息， 这是理解生物丰度调节的基础 转座因子在自然种群中的分布。

This project will continue investigations of the population properties of transposable elements of Drosphila. The question of whether or not the apparent fixation of certain elements at some chromosomal sites in a D. melanogaster population is real or artefactual will be answered. Some families of D. melanogaster elements used in previous studies will be characterized by restriction mapping. This will yield information essential for the interpretation of previous work, and for other proposed experiments. A study will be made of the rates of transposition and excision of several families of D. melanogaster elements. Southern blotting and in situ hybridization of element probes will be used to detect changes in element locations in isogenic stocks that have been maintained heterozygous and sheltered from recombination for over 100 generations will also be studied. Families of transposable elements from D. americana will be cloned. These will be used to determine whether elements have accumulated an the neo-Y chromosome of this species, as expected theoretically. The proposed experiments will provide information that is fundamental to an understanding of the regulation of the abundance of transposable elements in natural populations.