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POPULATION GENETICS OF TRANSPOSABLE ELEMENTS

转座元件的群体遗传学

基本信息

批准号：
3290312
负责人：
BRIAN CHARLESWORTH
金额：
$ 12.43万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-04-01 至 1989-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3290312
关键词：
Drosophilidae animal population genetics chromosome inversion gene frequency genetic crossing over genetic manipulation genetic mapping in situ hybridization molecular genetics natural selections nucleic acid probes sex chromosomes transposon /insertion element

项目摘要

There is now a well-established body of quantitative theory concerning the population genetics of transposable elements in sexual species. This theory predicts that element frequencies at chromosomal sites into which elements can insert should be low in natural populations. If selection acts to limit copy numbers, frequencies at X-linked sites will be lower than at autosomal sites. Furthermore, there will be a tendency for elements to accumulate preferentially in genomic regions where crossing over is absent. It is proposed to test these predictions in Drosophila melanogaster, using the technique of in situ hybridization of labeled probes of families of repeated DNA to polytene salivary chromosomes. Accumulation of elements with restricted crossing over will be tested by determining the abundance of elements in old-established, laboratory balanced inversion stocks, in rare naturally occurring inversions, and in regions where crossing over is normally absent such as chromosome 4 and the distal end of the X. Comparisons will be made with the abundance in suitable controls. Samples of X, 2nd, 3rd and 4th chromosomes from natural populations will be used to check the predictions of low element frequencies and a lower abundance of elements on the X relative to the autosomes.

现在有一套完善的量化理论体系，关于有性物种转座因子的群体遗传学。这理论预测，染色体位置上的元素频率在自然种群中可以插入的元素应该是低的。如果选择限制拷贝数量的行动，X链接网站的频率将会更低而不是在常染色体上。此外，将有一种趋势，即元素优先积累在基因组交叉区结束是缺席的。有人建议在果蝇身上测试这些预测，使用猪瘟病毒家系标记探针的原位杂交技术多线唾液染色体的重复DNA。元素的积累有限制的跨界将通过确定丰度来进行测试在过去建立的实验室平衡反转库存中的元素罕见的自然发生的倒置，在交叉是通常不存在，如4号染色体和X染色体的远端。将与适当控制措施的丰富性进行比较。样本来自自然种群的X、2、3和4号染色体将被用于检查低元素频率和较低丰度的元素的预测 X上的元素相对于常染色体。