Simultaneous Separation and Polymerization of Hazardous Organics via Enzyme Catalysts

这项建议涉及环境方面的重要任务， 去除某些有害的小有机分子（例如， 芳香胺、多氯联苯和多环芳香族化合物）， 一个独特的过程，利用酶催化在有机 相位 在该方法中，将水相与 在有机相中通过膜。 酶溶解在 有机相，并且膜被选择为具有 截止孔径，使有害的小有机分子， 而不是酶可以通过膜。 的 有机分子扩散到有机隔室中 酶将有机物聚合成高分子 随后可以从该反应器中除去的重物质 有机相通过沉降或超滤。 为了证明该工艺的可行性， (2CP)将被用作示例性有机溶剂，并且 聚合将在全氟萘烷中进行， 辣根过氧化物酶作为酶催化剂。 这种催化作用 需要由氢提供的自由基 过氧化物，本身由乙醇氧化催化产生， 醇氧化酶

This proposal addresses the environmentally important task of removing certain hazardous small organic molecules (e.g. aromatic amines, PCBs and polycyclic aromatics) from water by a unique process utilizing enzyme catalysis in an organic phase. In this process, the aqueous phase is separated from in organic phase by a membrane. The enzyme is dissolved in the organic phase, and the membrane is selected to have a cutoff pore size so that the hazardous small organic molecule, but not the enzyme, can pass through the membrane. The organic molecule diffuses into the organic compartment where the enzyme polymerizes the organic into a high molecular weight species which can subsequently by removed from the organic phase via settling or ultrafiltration. To demonstrate the feasibility of this process, 2 chlorophenol (2CP) will be used as the example organic solvent, and the polymerization will take place in perfluorodecalin with horseradish peroxidase as the enzyme catalyst. This catalysis requires free radicals which will be provided by hydrogen peroxide, itself generated by alcohol oxidation catalyzed by alcohol oxidase.