Ozone-induced graft polymerization of glycidyl methacrylate for preparation of polymer microspheres with a potential of separation and purification of polyunsaturated fatty acids

在前期研究的基础上，我们提出了对二十二碳六烯酸(DHA)和二十碳五乙酸(EPA)等多不饱和脂肪酸具有亲和力的聚合物微球的制备方法。本研究设计了表面含环氧基团的聚合物微球的亲和力合成路线，为制备对多不饱和脂肪酸具有较高吸附潜力的聚合物微球进行了新的设计。也就是说，通过臭氧引发甲基丙烯酸缩水甘油酯在分散聚合制备的单分散聚苯乙烯微球上接枝聚合，制备了具有更多吸附Ag+中心的聚合物微球。为了使聚合物微球在臭氧气氛中均匀分散，采用了四块挡板的烧瓶。…此外，在设备中暴露出聚合物微球后，甲基丙烯酸缩水甘油酯在密封玻璃管或四颈圆底烧瓶中进行接枝聚合。其次，研究了工艺参数对聚苯乙烯微球接枝聚合物层形成的影响。本研究以臭氧暴露处理时间、接枝聚合时间、聚合温度、单体浓度、聚合介质等为工艺参数，进行了实验研究。实验发现，臭氧处理时间和接枝聚合时间促进了聚甲基丙烯酸缩水甘油酯颗粒层的形成，而聚合介质的变化对其影响不大。结果表明，甲基丙烯酸缩水甘油酯的适宜浓度为5%，单体的加入越多，溶液中聚甲基丙烯酸缩水甘油酯微球的形成越明显。较少

From our previous research, we proposed the preparation method of polymer microspheres with an affinity for polyunsaturated fatty acids such as docosahexaenoic acid (DHA) and eicosapentaacetic acid (EPA). In that study, the synthesis route to endow affinity to polymer microspheres bearing epoxy group on their surface was designed.In this research, we planned a new design for the preparation of polymer microspheres with higher potential on adsorption of polyunsaturated fatty acids. That is to say, the experiment was conducted to prepare polymer microspheres which had more sites for Ag+ adsorption by ozone-induced graft polymerization of glycidyl methacrylate on monodispersed polystyrene microspheres produced by dispersion polymerization.At first, the equipment for ozone exposure of the prepared polymer microspheres was constructed using an advanced rotary evaporator reactor. Flask with four baffle plates was adopted in order polymer microspheres to be well-dispersed in ozone atmosphere. … More After the exposure of polymer microsphere in the equipment, the graft polymerization of glycidyl methacrylate was conducted in a sealed glass tube or a four-necked round bottom flask. As a result, the standard recipe and protocol was determined.Secondly, we tried to clarify the effect of process parameters on formation of grafted polymer layer on polystyrene microspheres. In this study, we varied the parameter of treatment time of ozone-exposure, graft polymerization time, polymerization temperature, monomer concentration and polymerization medium as process parameters. It was found from this experiment that the treatment time of ozone-exposure and graft polymerization time enhanced the formation of grated poly(glycidyl methacrylate) layer, whereas no change was observed by change of polymerization medium. The adequate concentration of glycidyl methacrylate was found to be 5 vol% and more addition of monomer induced the formation of poly(glycidyl methacrylate) microspheres in solution simultaneously. Less