Regulation of ribosomal RNA synthesis by fructose-1, 6-biophosphate

调节蛋白质生物合成机制的水平以响应生长条件对每个活细胞来说都是至关重要的。在大肠杆菌中，有两个不同的调控系统将核糖体RNA(RRNA)的合成速度与生长速度结合在一起。然而，将细胞的中央新陈代谢与rRNA合成速率联系起来的实际机制(S)仍然完全不清楚。观察到1，6-二磷酸果糖(FBP)含量的增加优先抑制了rRNA合成的中枢代谢调节。FBP专门抑制rRNA合成的粗转录-翻译系统的开发为剖析这一效应提供了一个实验系统。确定是否需要单独的RNA聚合酶或粗提物中的其他因素来抑制的实验将确定FBP的作用目标。对这种相互作用的遗传分析将建立与FBP相关的调节回路起作用的生长条件。这个项目提供了一个机会来理解一个将rRNA合成与碳代谢联系在一起的分子电路，这是一个对每个活着的有机体都至关重要的问题，而这个问题在任何实验系统中都没有被阐明。

Modulating the level of protein biosynthetic machinery in response to growth conditions is of utmost importance to every living cell. In E. coli, two different regulatory systems couple the rate of ribosomal RNA (rRNA) synthesis to growth rate. However, the actual mechanism(s) linking the central metabolism of the cell to the rate of rRNA synthesis remain completely unknown. The observation that increased amounts of fructose 1,6 biphosphate (FbP) preferentially inhibits in connecting central metabolism regulation of rRNA synthesis. The development of a crude transcription-translation system in which FbP specially inhibits rRNA synthesis provides an experimental system for dissecting this effect. Experiments to determine whether RNA polymerase alone or additional factors in the crude extract are needed for inhibition will determine the target of FbP action. Genetic analysis of this interaction will establish the growth conditions under which the regulatory circuit involving FbP is operative. %%% This project offers a chance to understand a molecular circuit that couples rRNA synthesis to carbon metabolism, a problem of central importance to every living organism that has not been elucidated in any experimental system.