Do Gill from Symbiont-free Mussels Gain Sufficient Cellular Energy from Sulfide Oxidation to Support Cellular Work?

硫化氢是海洋沉积物中自然产生的， 对大多数生物都是有毒的然而，一些动物从 富含硫化物的环境避免了这种硫化物中毒 细胞水平的解毒反应，这些氧化 反应可能是潜在有用的能源。的目标 这个项目是为了确定硫化物居民是否获得足够的 来自硫化物氧化的细胞能量以支持细胞工作。 实验组织的选择将是纤毛鳃从两个 一种来自富含硫化物的环境， 一个来自无硫化物环境。鳃是一种勤劳的组织 作为滤食性动物之间的主要界面 和他们的环境。在硫化物存在和不存在的情况下，我们 将测量细胞能量代谢的几个参数， 包括线粒体的能量产生和能量 鳃纤毛的消耗。这项研究将开始解决 关于管理机制的基本问题， 协调细胞能量消耗与能量生产; 分子和细胞机制，使生存在自然 紧张的栖息地;和硫化物栖息地的入侵 海洋无脊椎动物可能导致了 无脊椎动物宿主和硫化物氧化之间的共生关系 细菌本报告将讨论与下列方面的贡献有关的问题： 硫化物系统的河口总生产力。

Hydrogen sulfide, which is naturally produced in marine sediments, is poison to most living organisms. However, some animals from sulfide-rich environments avoid this poisoning with sulfide detoxification reactions at the cellular level, and these oxidation reactions may be a potentially useful source of energy. The goal of this project is to determine if sulfide inhabitants gain sufficient cellular energy from sulfide oxidation to support cellular work. The experimental tissue choice will be ciliated gills from two species of marine mussels, one from sulfide-rich environments and one from sulfide-free environments. Gills are hard-working tissues that act as the primary interface between filter-feeding animals and their environment. In the presence and absence of sulfide, we will measure several parameters of cellular energy metabolism, including energy production by the mitochondria and energy consumption by the gill cilia. This research will begin to address basic questions concerning the regulatory mechanisms that coordinate cellular energy consumption with energy production; the molecular and cellular mechanisms that allow survival in natural stressful habitats; and the invasion of the sulfide habitat by marine invertebrates that may have led to the eventual development of the symbiosis between invertebrate hosts and sulfide-oxidizing bacteria. It will address questions concerning the contribution of sulfide systems to the total productivity of estuaries.