How Mussel Gill Mitochondria Choose Between Sulfide and Endogenous Carbon Substrate as a Source of Electrons for ATP Production

贻贝鳃线粒体如何选择硫化物和内源碳底物作为 ATP 生产的电子源

• 批准号: 9728409
• 负责人: Jeannette Doeller
• 金额: $ 16万
• 依托单位: University of Alabama at Birmingham
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728409&HistoricalAwards=false
• 关键词: How; Mussel; Gill; Mitochondria; Choose

Deoller 9728409 Hydrogen sulfide, a common constituent of several natural and man-made aquatic habitats, is both a potent toxin of aerobic cellular respiration and an electron-rich molecule used by some bacteria as an energy source. In ciliated gills from Geukensia demissa, a marine mussel from sulfide rich sediments, sulfide oxidation supports cellular work, indicating for the first time that in an intact metazoan tissue, mitochondria switch from carbon catabolism to sulfide oxidation for energy production. In this project, using excised intact G. demissa gills and isolated gill mitochondria, three central questions concerning the transition from carbon catabolism to sulfide oxidation will be addressed: what is the nature of this transition; what factors regulate it; and, since sulfide exposure history influences capacity for sulfide oxidation, what mechanism(s) underlies this alternation in capacity? The results of this work will help rigorously address a long-standing question, namely how do organisms adapt to sulfide exposure? The findings may indicate that energy budgets of ecosystems containing sulfide habitats need reconsideration if sulfide oxidation can result in carbon sparing in metazoans. Assessment of mitochondrial function using innovative and state-of-the-art techniques will further our understanding of this possibly widespread adaptation to seemingly in hospitable environments.

德奥勒9728409 硫化氢是几种天然和人造水生栖息地的常见成分，既是有氧细胞呼吸的强效毒素，也是一些细菌用作能源的富电子分子。 在Geukensia demissa（一种来自富含硫化物的沉积物的海洋贻贝）的纤毛鳃中，硫化物氧化支持细胞工作，这首次表明在完整的后生动物组织中，线粒体从碳催化剂转换为碳催化剂。 到硫化物氧化用于能源生产。 本研究利用切除的完整G. demissa鳃和分离的鳃线粒体， 三个核心问题 关于 将讨论从碳催化剂到硫化物氧化的过渡：这种过渡的性质是什么;什么因素调节它;以及，由于硫化物暴露历史影响硫化物氧化的能力，这种能力变化的基础是什么机制？ 这项工作的结果将有助于严格解决一个长期存在的问题，即生物体如何适应硫化物暴露？ 研究结果可能表明，如果硫化物氧化，含硫化物生境的生态系统的能量收支需要重新考虑， 会导致后生动物的碳节约。 使用创新和最先进的技术评估线粒体功能将进一步加深我们对 这 可能广泛适应 似乎在适宜的环境中。