Structure and Function of Regulatory Proteins

拟议的研究涉及的化学机制， 在NIE-115细胞中表达的兴奋性5-羟色胺3型受体。 将采用两种快速动力学方法：（1）细胞流动 方法，和（2）激光脉冲光解不稳定的5-羟色胺笼 物种 将研究跨膜通道行为， 时间和血清素浓度变化范围很大 的影响 将探索抑制剂。 %%% 神经递质受体介导的反应在特定细胞的 神经系统负责启动、调节和 信号传输的调制。 这些过程都是 神经系统功能所必需的。 拟议的研究包括 旨在发展一个完整的动力学的理解， 配体（5-羟色胺）与其受体之间的相互作用， 选择细胞膜。 取决于血清素是否与 以及在多长时间内，受体形成瞬时离子- 传导跨膜通道，提供基本的 信号传输事件。 仔细的时间依赖性研究 为了开发有用的信号传输模型。 发展这种基本信息是取得进展的根本， 了解神经系统功能的本质， 一体化

The proposed studies address the chemical mechanism of the excitatory serotonin type-3 receptor as expressed in NIE-115 cells. Two rapid kinetic approaches will be employed: (1) a cell-flow method, and (2) laser-pulse photolysis of labile serotonin cage species. Transmembrane channel behavior will be investigated over wide ranges of time and serotonin concentration. The effects of inhibitors will be explored. %%% Neurotransmitter receptor-mediated reactions in specific cells of the nervous system account for the initiation, regulation, and modulation of signal transmission. These processes are all required for nervous system function. The studies proposed are aimed at developing a complete kinetic understanding of the interaction between a ligand (serotonin) and its receptor in selected cell membranes. Depending on whether serotonin is bound to the receptor and for how long, the receptor forms transient ion- conducting transmembrane channels which provide the fundamental signal transmission event. Careful time-dependence studies are required in order to develop useful models of signal transmission. Development of such basic information is fundamental to progress in understanding the nature of nervous system function and integration.