RUI: Acquisition of equipment for tracking and purifying radioactive proteins

本提案的目的是获得高质量， 自动FPLC系统和一个新的伽马计数器，以纯化和 跟踪蛋白质/肽（特别是放射性的 蛋白质/肽）和它们的金属或辅基。 需要FPLC系统，因为 主要研究者目前只有非常有限的 进入这些系统，虽然在每一个，净化 蛋白质/肽（为了确定它们的结构， 功能和调节）是一项重要的活动。 一个新的伽玛 需要计数器，因为现有计数器为12年 年纪大了，可能会变得不可靠。 如果它应该 失败，这将大大限制研究的主要 调查人员，他们完全依赖它来追踪 生物系统中的铜和铁;跟踪铜和铁 铁结合蛋白及其受体 纯化和/或在其结合能力的研究中， 动力学和功能;确定 特异性酶的底物和辅因子 标签。 使用的同位素（67 Cu，64 Cu，59 Fe，125 I）均为 γ发射体，并且通常需要双重标记。

The objective of this proposal is to acquire a high quality, automatic FPLC system and a new gamma counter, to purify and track proteins/peptides (particularly radioactive proteins/peptides) and their metals or prosthetic groups. The FPLC system is needed because the laboratories of the primary investigators currently have only very limited access to such systems, although in each, purification of proteins/peptides (for determination of their structure, function and regulation) is a major activity. A new gamma counter is needed because the existing one is twelve years old and threatening to become unreliable. If it should fail, this would greatly limit the research of the primary investigators, who are utterly dependent upon it for tracing copper and iron in biological systems; tracking copper and iron binding proteins and their receptors during their purification and/or in studies of their binding capacities, kinetics and functions; identifying the binding sites of substrates and cofactors of specific enzymes via affinity labeling. The isotopes used (67Cu,64Cu,59Fe, 125I) are all gamma emitters, and double labeling is often required.