Analysis of Mechanisms in Stimulous-Secretion Coupling

WPC 2 * * * * * * * * * * * * * * * * * * * *；X @ HP Laserjet （25-in- in, Network）PRS x @, t 0 NX @ 2 B ~ # x 6 x @ 8；这个项目的长期目标是了解在各种刺激事件下控制细胞分泌激素的分子机制。这些机制，目前正在被实验学家发现，涉及振荡和波动的复杂现象，需要使用数学和计算分析来理解。特别感兴趣的是1)分泌细胞中钙振荡和波的控制机制；2)激动剂刺激G蛋白事件的机制，特别是对胰腺β细胞离子通道的控制；3)葡萄糖代谢控制下胰腺分泌细胞胰岛素分泌的2 ~ 5分钟周期钙振荡机制；4)垂体前叶促性腺激素的电活动和钙水平调控机制；5)体外胰岛胰岛素分泌15 - 30周期振荡的机制。主要研究人员采用的方法涉及开发和分析与实验发现的机制相对应的常微分方程和偏微分方程。关于这五种机制的速率有足够的定量资料来证明这些计算是合理的，这些计算将继续与实验工作密切结合。计算工作将在并行架构平台上完成。这个建议是由细胞生物学，计算生物学和应用数学项目共同支持的。＊＊＊

WPC 2 B R V ` Z #| x Courier 10cpi 2 x x x , x @ 8 ; X @ HP Laserjet (25-in-one, Network) HPLASEII.PRS x @ , t 0 NX @ 2 B ~ # x 6 X @ 8 ; X @# ( 9300799 Keizer The long term objective of this project is to understand the molecular mechanisms that control secretion of hormones from cells in response to various types of stimulatory events. These mechanisms, which are currently being uncovered by experimentalists, least to complex phenomena involving oscillations and waves that require the use of mathematical and computational analysis for their understanding. Of particular interest are 1) the mechanisms controlling calcium oscillations and waves in secretory cells; 2) the mechanism involved in agonist stimulation of G protein events, especially, control of ion channels in the pancreatic beta cell; 3) the mechanisms underlying the 2 5 minute period calcium oscillations in insulin secretion from pancreatic secreting cells that are under the control of glucose metabolism; 4) the mechanisms involved in the control of electrical activity and calcium levels in anterior pituitary gonadotrophs; and 5) the mechanisms responsible for the 15 30 period oscillations in insulin secretion from pancreatic islets in vitro. The method employed by the principal investigator involve the development and analysis of ordinary and partial differential equations corresponding to mechanisms uncovered by experiment. Sufficient quantitative information regarding the rates of these five mechanisms is available to justify these calculations, which will continue to be interfaced closely with experimental work. The computational work will be done on a parallel architecture platform. This proposal is being jointly supported by the Cell Biology, the Computational Biology, and the Applied Mathematics programs. ***